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| 抗DR5单抗CTB006联合5-FU对结直肠癌细胞系作用研究 | |
| 引用本文： | 高旭,周正,沈恩允,景晓娟,郭雷鸣,王仙斌,孙劲文,杜云峰. 抗DR5单抗CTB006联合5-FU对结直肠癌细胞系作用研究[J]. 中国医药生物技术, 2011, 6(2): 106-110. DOI: 10.3969/cmba.j.issn.1673-713X.2011.02.005 |
| 作者姓名： | 高旭 周正 沈恩允 景晓娟 郭雷鸣 王仙斌 孙劲文 杜云峰 |
| 作者单位： | 1. 华北煤炭医学院研究生部,唐山,063000 2. 煤炭总医院普外肿瘤科,北京,100028 3. 北京同为时代生物技术有限公司,100176 4. 中国人民解放军总参谋部总医院内分泌科,北京,100031 |
| 摘 要： | 目的探讨肿瘤坏死因子相关诱导配体受体（DR5）激动型单抗CTB006对人结直肠痛细胞系SW1116、SW480、SW620、Colo205的影响。方法采用ATPlite法检测CTB006组、5-FU组及两药物合用组作用后的细胞存活率，流式细胞仪技术检测细胞系表面DR5的表达水平，研究两者之间的关系。结果CTB006和5-FU对四种结直肠癌细胞增殖均表现出抑制作用。CTB006对早期细胞SW1116增殖抑制效果欠佳，对中晚期细胞SW480、SW620、Colo205有着良好的增殖抑制作用（P〈0．05）。当联合使用5-FU后，对后三者有明显的协同作用。流式细胞仪检测DR5表达水平，SW1116细胞表达（47．01±30．4）％，SW480细胞表达（76．11±15．1）％，SW620细胞表达（86．77±9.3）％，Col0205细胞表达（93．55±7．9）％。结论CTB006对中晚期结直肠癌杀伤作用较强，联用5-FU具有显著的协同作用。 |
| 关 键 词： | 结直肠肿瘤 受体,TINT相关凋亡诱导配体 药物协同作用 氟尿嘧啶 |
Effects of the combination of anti-human DR5 monoclonal antibody and 5-Fluorouracil on the proliferation of colorectal cancer cells in vitro |
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| GAO Xu,ZHOU Zheng,SHEN En-yun,JING Xiao-juan,GUO Lei-ming,WANG Xian-bin,SUN Jin-wen,DU Yun-feng. Effects of the combination of anti-human DR5 monoclonal antibody and 5-Fluorouracil on the proliferation of colorectal cancer cells in vitro[J]. Chinese Medicinal Biotechnology, 2011, 6(2): 106-110. DOI: 10.3969/cmba.j.issn.1673-713X.2011.02.005 | |
| Authors: | GAO Xu ZHOU Zheng SHEN En-yun JING Xiao-juan GUO Lei-ming WANG Xian-bin SUN Jin-wen DU Yun-feng |
| Affiliation: | GAO Xu,ZHOU Zheng,SHEN En-yun,JING Xiao-juan,GUO Lei-ming,WANG Xian-bin,SUN Jin-wen,DU Yun-feng Graduate Department of North China Coal Medical University,Tangshan 063000,China,Oncology and General Surgery Department,Coal General Hospital,Beijing 100028,Beijing Cotimes Biotechnology Co.,Ltd,Beijing 100176,Endocrinology Department of General Hospital of PLA General Staff Headquarters,Beijing 100091 |
| Abstract: | Objective To approach the effects of the combination of tumor necrosis factor-related inducing ligand receptor(DR5) monoclonal antibody CBT006 and 5-Fluorouracil(5-FU) on the proliferation of human colorectal cancer cells.Methods ATPlite assay was used to detect the influences of CBT006 and 5-FU alone or in combination on the proliferation of human colorectal cancer cells including SW1116,SW480,SW620 and Colo205 in different pathological stages.The expression of cell surface DR5 levels were examined by usin... |
| Keywords: | Colorectal neoplasms Receptor TNF-related apoptosis-inducing ligand Drug synergism Fluorouracil |
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