尖锐湿疣患者Th1/Th2细胞因子的检测

目的 检测尖锐湿疣(CA)患者CD3⁺T细胞分泌细胞因子的水平,分析Th1细胞因子和Th2细胞免疫活动。方法 首先用刺激物刺激细胞,增加细胞内细胞因子的表达,然后加入荧光标记的特异性抗细胞因子单克隆抗体,特异性抗原抗体结合,最后应用流式细胞仪分析特异性细胞因子表达水平。结果 CA患者Th1型细胞因子干扰素γ、白介素2、肿瘤坏死因子α、白介素12表达水平较正常对照显著降低,Th2型细胞因子白介素10表达水平较正常人对照差异无显著性。结论 CA患者Th1型反应模式处于弱势状态,相对地Th2型反应模式处于优势状态,Th1/Th2平衡失调,Th1/Th2平衡向Th2方向漂移。CA患者体内Th2型细胞因子模式占优势状态,可能是病毒发生免疫逃逸引起免疫损伤的一种机制。

Detection of Th1/Th2 Cytokines in the Peripheral Blood Lymphocytes of Patients with Condyloma Acuminatum

Objective To detect cytokine levels secreted by CD3⁺T cells and monitor the Th cell imbalance in the patients with condyloma acuminatum(CA).Methods The peripheral blood lymphocytes were isolated and stimulated prior to analysis.Cytokine detection was performed using monoclonal antibodies,which were fluorochrome-conjugated for easier direct manipulation in flow cytometry analysis.The results were pre-sented as cytograms showing dual staining of lymphocytes.Results The levels of IL-2,IL-12,IFN-γ and TNF-α produced by Th1cells decreased,while the level of IL-10 produced by Th2 cells had no significant change in CA patients in comparison with healthy controls.Conclusions There is Th1/Th2 imbalance in CA patients with a decline of Th1type cytokines.The Th1/Th2 imbalance might be the mechanism that HPVs escape from immunosurveillance in CA patients.