人子宫内膜腺癌细胞系的建立

目的构建人子宫内膜腺癌细胞系。方法将1例高分化子宫内膜腺癌手术切除的肿瘤组织制成单细胞悬液,用含10%胎牛血清的DMEM/F-12培养基进行原代培养,倒置显微镜下观察细胞形态,并用免疫荧光染色法检测其角蛋白。结果体外培养细胞传至50代以上,传代后细胞单层贴壁,散在生长,密度增加时见堆积现象,失去接触抑制,细胞质中角蛋白表达阳性。结论成功建立了人子宫内膜腺癌细胞系。

Establishment of human endometrial adenocarcinoma cell line

Objective To establish human endometrial adenocarcinoma cell line.Methods Tumor tissues from 1 case with well differentiated endometrial adenocarcinoma were made into single-cell suspension and cultured in DMED/F-12 medium supplemented with 10% fetal bovine serum.The morphology of the cells was observed by inverted microscope.The immunofluorescent staining technique was used to detect cytokeratin in endometrial carcinoma cells.Results The cells were subcultured more than 50 generations,monolayer cultured cells were polygonal in shape and had a tendency to pile up without contact inhibition.The expression of cytokeratin was positive in the cells.Conclusion The novel endometrial adenocarcinoma cell line is successfully established.