化痰消瘤方抗Lewis荷瘤小鼠肺癌转移及对CD44、CD44v6、E-cad表达的影响

Effect of Huatan Xiaoliu Decoction on Tumor Metastasis,and Expressions of CD44, CD44v6 and E-cad in Lewis Tumor-Bearing Mice

Objective:To study the effect of anti-metastasis and expressions of CD44,CD44v6 and E-cad of Huatan Xiaoliu Decoction in Lewis lung cancer,and to observe the mechanism of restraining tumor growth and tumor metastasis.Methods:The experimental animal Lewis lung carcinoma were randomly divided into low,medium and high dose,CTX and blank groups.After 14 days,the experiment was over and the mice were killed.And the tumor was stripped and weighed to calculate the inhibition rate.The weight of lung in mice were counted to calculate the rate of anti-lung metastasis.To study the effects of Huatan Xiaoliu Decoction on tumor metastasis and expressions of CD44,CD44v6 and E-cad in Lewis lung carcinoma by RT-PCR and Western blot.Results:In comparison with blank group,in terms of weights of tumors and pulmonary metastasis focuses,the low-dose group of Huatan Xiaoliu Decoction showing no statistical differences(P ＞ 0.05),the medium and high dose groups of Huatan Xiaoliu Decoction,CTX groups showing statistical difference (P ＜ 0.05).The results of RT-PCR indicating that,in comparison with blank group,the expression of CD44 mRNA and CD44v6 mRNA was low(P ＜ 0.01),the expression of E-cad was high (P ＜ 0.01).The expression of CD44,CD44v6 and E-cad were also detected by Western blot.And the results were similar to former.Conclusion:Huatan Xiaoliu Decoction has the function to restrain tumor growth and tumor metastasis in dose-dependent manner.The underlying mechanisms are possibly related to the increasing of the expression of E-cad.It could raise adhesion among tumor cells and weaken the ability of invasion to surrounding tissue by tumor cells.At the same time,the mechanism also relates to the decreasing in expression of CD44 and CD44v6.It could reduce the adhesion between tumor cells and ECM and inhibit the ability of invasion and metastasis of tumor cells.