中药方剂配以音乐疗法对肝郁证大鼠的改善作用

目的:研究中药方剂配以音乐疗法对肝郁证大鼠的作用。方法:取80只Wistar大鼠随机分为空白组、模型组、音乐组、中药方剂组和中药方剂+音乐组。除空白组外,各组接受21 d不可预知的慢性应激刺激,造模结束后,中药方剂组和中药方剂+音乐组给予5.0 g·(kg·d)~(-1)药物,其余各组给予等体积的生理盐水溶液。与此同时,除空白组和模型组,其余各组在安静环境下播放α脑波音乐1 h,3次·d-1。末次给药1 h后,腹主动脉取血,测定血清体积及血浆促肾上腺皮质激素(adrenocorticotrophic hormone,ACTH)和血清皮质酮(corticosterone,CORT)值,同时称取主要脏器质量并分析主要脏器系数。结果:与模型组比较,中药方剂组和中药方剂+音乐组大鼠血清体积比、肝脏系数、脾脏系数、血浆ACTH值和血清CORT值显著降低(P0.05);与模型组比较,音乐组大鼠肝脏、肾脏系数显著降低(P0.05),血清体积比、血浆ACTH值和血清CORT值差异不显著(P0.05)。结论:中药方剂配以音乐疗法对肝郁证大鼠有一定的改善作用。

Improvement Study on Chinese Medicine Formulae in Combination with Music Therapy in Rats with Liver Depression Syndrome

Objective:To investigate the effect of Chinese medicine formulae plus music therapy in rats with liver depression syndrome.Methods:80 Wistar rats were divided into 5 groups randomly,normal group,model geoup,music group,Chinese medicine formulae group and Chinese medicine formulae plus music therapy group.In addition to the normal group,each group received unpredictable chronic stress stimulation in 21 days.After the building groups of animals at the end of the time,Chinese medicine formulae group and Chinese medicine formulae plus music therapy group were treated with medicine at a dose of 5.0 g/(kg · d)-1 The other groups were treated with physiological saline.Meanwhile,all the rats except normal group and model group were compelled to listen to the alfa music in a quiet environment for 1 h once and 3 times a day.The last 1 h after the treatment,the blood was taken from abdominal aorta,serum volume,adrenocorticotrophic hormone (ACTH) and corticosterone (CORT) were measured and main organ coefficients were analyzed.Results:Compared with the model group,serum volume ratio,liver index,spleen index,plasma ACTH and serum CORT value of rats decreased significantly in the groups of Chinese medicine group and traditional Chinese medicine plus music (P ＜0.05);compared with the model group,the liver and kidney coefficient rats decreased significantly(P ＜ 0.05) and serum volume ratio,plasma ACTH value and the value of CORT in serum had no significant difference in the music group(P ＞ 0.05).Conclusion:The method of Chinese medicine formulae plus music therapy has certain meliorative effect on liver depression rats.