钩状石斛化学成分的研究

 目的分离鉴定钩状石斛中的化学成分。方法体积分数为95%乙醇提取,硅胶柱色谱分离,用IR,MS,¹H-NMR,¹³C-NMR和HMBC等方法确定结构。结果分得9个化合物,分别为对羟基顺式肉桂酸三十烷基酯(n-triacontyl p-hydroxy-cis-cin-namate,Ⅰ),对羟基反式肉桂酸三十烷基酯(defuscin,Ⅱ),β-谷甾醇(β-sitosterol,Ⅲ)、aduncin(Ⅳ)、dengibsinin(Ⅴ)、dengibsin(Ⅵ)、chrysotoxone(Ⅶ)、4-羟基-3-甲氧基苯甲酸(4-hydroxy-3-methoxybenzoicacid,Ⅷ)和胡萝卜苷(daucosterol,Ⅸ)。结论4-羟基-3-甲氧基苯甲酸为首次从石斛属植物中分离得到,化合物Ⅰ~Ⅲ,Ⅴ~Ⅸ均为首次从该种植物分得,化合物Ⅳ和Ⅴ的碳谱数据为首次报道。

Study on Chemical Constituents of Dendrobium aduncum

OBJECTIVE To isolate and elucidate the constituents from Dendrobium aduncum Hook. METHODS The chromatography on silica gel was used for the isolation and purification of the constituents. The structures were determined by IR,MS,¹H-NMR,¹³C-NMR and HMBC spectral analysis.RESULTS Nine compounds were obtained and identified as following: n-triacontyl p-hydroxy-cis-cinnamate(Ⅰ),defuscin(Ⅱ),β-sitosterol(Ⅲ),aduncin(Ⅳ),dengibsinin(Ⅴ),dengibsin(Ⅵ),chrysotoxone(Ⅶ),4-hydroxy-3-methoxy benzoic acid(Ⅷ) and daucosterol(Ⅸ).CONCLUSION The compound of 4-hydroxy-3-methoxy benzoic acid is firstly isolated from Genus Dendrobium and Compound Ⅰ～Ⅲ, Ⅴ～Ⅸ are found from this species for the first time,The ¹³C-NMR data of compound Ⅳ and Ⅴ are reported for the first time.