TMPO基因表达对肺癌患者临床预后的影响

 目的 探讨TMPO基因对肺癌患者预后的影响。方法 检索并下载NCBI的肿瘤公共数据集,分析肺癌基因表达谱资料及临床信息,明确TMPO基因在正常肺组织和肺癌组织中的表达差异,并利用基因集富集分析方法(GSEA)进一步探讨TMPO基因对肺癌细胞的具体作用机制。结果 TMPO基因在肺癌组织中的相对表达值为0.254 ±0.594,明显高于在正常肺组织中的相对表达值-0.332 ±0.285(P<0.0001 )。TMPO基因高表达肺癌患者的TNM分期明显高于TMPO低表达的肺癌患者(P<0.0001, P<0.0001, P=0.031), TMPO低表达组肺癌患者的无进展生存期(P<0.0001)、总生存期(P<0.0001)结果均优于TMPO高表达组的肺癌患者。TMPO高表达样本富集了与DNA修复、MYC信号通路1、有丝分裂、细胞周期G2M检查点、精子发生、E2F信号通路、MYC信号通路2、mTORC1信号通路相关的基因集。结论 TMPO基因在多种肿瘤中高表达,其通过多种途径来促使肿瘤细胞增殖,进而影响肿瘤患者的临床预后,因此TMPO基因可以作为诊疗肺癌的标志物和潜在靶点。

Significance of TMPO forprognosis of patients with lung cancer

Objective To clarify the relationships between TMPO gene expression and clinical prognosis of lung cancer.Methods NCBI datasets were collected while expression profiles and clinical information were analyzed to characterize the expressions of TMPO gene in lung cancer patients and adjacent normal lung tissues.The correlations between TMPO expressions and TNM staging,progression free survival and overall survival of lung cancer patients were studied.Gene set enrichment analysis was applied to investigate the exact mechanisms related to the expression of TMPO and the proliferation of lung cancer cells.Results The level of TMPO exprcssion was higher in lung cancer patients than that in normal controls (P ＜ 0.0001).Lung cancer patients with higher expressions of TMPO were associated with better TNM staging(P ＜ 0.0001,P ＜ 0.0001,P =0.031),progression free survival (P ＜ 0.0001),and overall survival(P ＜ 0.0001)compared with TMPO low expression cohort.The result of GSEA indicated that TMPO might promote the proliferation of lung cancer cells involving DNA repair,MYC signaling,mitotic spindle,G2 M checkpoint,spermatogenesis,E2F signaling and mTORC1 signaling.Conclusions Our results suggest that TMPO might be an independent risk factor for lung cancer patients.