|
|||||
|
|
| 人白介素- 4对牛血管内皮细胞保护作用的研究 | |
| 引用本文： | 陈刚,傅志仁,王梁华,焦炳华. 人白介素- 4对牛血管内皮细胞保护作用的研究[J]. 细胞与分子免疫学杂志, 2005, 21(6): 775-777 |
| 作者姓名： | 陈刚 傅志仁 王梁华 焦炳华 |
| 作者单位： | 1. 成都军区昆明总医院肝胆外科,云南,昆明,650032 2. 第二军医大学长征医院器官移植中心,上海,200003 3. 第二军医大学生物化学与分子生物学系,上海,200433 |
| 摘 要： | 目的:研究人白介素-4(hIL-4)对经TNF-α活化的牛主动脉内皮细胞(BAEC)的保护作用。方法:用不同浓度的hIL-4与BAEC共孵育2h后,再与4μg/L的TNF-α共孵育6h或18h。应用细胞ELISA方法,检测BAEC表面的E选择素和ICAM-1的表达;用MTT比色法测定hIL-4对BAEC活性的影响。结果:在一定浓度内用hIL-4预处理BAEC后,能明显抑制TNF-α诱导活化BAEC上的E选择素与ICAM-1的表达,并呈现一定的剂量依赖性。用MTT比色法测定BAEC活性的实验表明,各实验组BAEC的活性与对照组无明显差异性。结论:hIL-4能明显抑制TNF-α诱导活化的BAEC表达E选择素与ICAM-1;hIL-4对BAEC的正常功能具有一定的保护作用。 |
| 关 键 词： | 人白介素-4 牛主动脉内皮细胞 E选择素 细胞间黏附分子-1 |
| 文章编号： | 1007-8738（2005）06-0775-03 |
| 收稿时间： | 2004-11-29 |
| 修稿时间： | 2005-04-28 |
The effect of hIL-4 on the expression of E-selectin and ICAM-1 on bovine aortic endothelial cells |
|
| CHEN Gang,FU Zhi-ren,WANG Liang-hua,JIAO Bing-hua. The effect of hIL-4 on the expression of E-selectin and ICAM-1 on bovine aortic endothelial cells[J]. Chinese journal of cellular and molecular immunology, 2005, 21(6): 775-777 | |
| Authors: | CHEN Gang FU Zhi-ren WANG Liang-hua JIAO Bing-hua |
| Affiliation: | 1.Department of Hepatic and Biliary Surgery, Kunming General Hospital, Kunming 650032; 2.Organ Transplantation Center, Changzheng Hospital, Second Military Medical University, Shanghai 200003 ; 3. Department of Biochemistry and Molecular Biology, Second Military Medical University, Shanghai 200433, China |
| Abstract: | AIM: To investigate the effect of human interleukin-4 (hIL-4) on the expression of E-selectin and ICAM-1 on bovine aortic endothelial cells(BAEC) activated with TNF-alpha. METHODS: BAEC were cultured in different concentrations of hIL-4 for 2 h followed by treatment with 4 microg/L TNF-alpha for 6 h or 18 h. The expression of E-selectin and ICAM-1 on BAEC was detected by cell-ELISA. The effect of hIL-4 on viability of BAEC was detected by MTT colorimetry. RESULTS: Pretreatment of BAEC with hIL-4 inhibited the expression of E-selectin and ICAM-1 on BAEC activated by TNF-alpha in a dose-dependent manner (P<0.01). Both TNF-alpha and hIL-4 had no effect on the viability of BAEC. CONCLUSION: hIL-4 can suppress the expression of E-selectin and ICAM-1 on BAEC activated by TNF-alpha, which may contribute to the xenotransplant immune tolerance associated with hIL-4. |
| Keywords: | human interleukin-4 bovine aortic endothelial cells E-selectin intercellular adhesion molecule-1 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |