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| 胰岛素样生长因子1对肾间质纤维化模型大鼠的实验研究 | |
| 引用本文： | 张宏,王丙增,张旭亚,孟凡威. 胰岛素样生长因子1对肾间质纤维化模型大鼠的实验研究[J]. 医学综述, 2013, 19(5): 917-918,921 |
| 作者姓名： | 张宏 王丙增 张旭亚 孟凡威 |
| 作者单位： | 河南大学第一附属医院儿科,河南,开封,475001 |
| 基金项目： | 河南省教育厅自然科学基金，河南大学自然科学基金 |
| 摘 要： | 目的探讨胰岛素样生长因子1(IGF-1)与肾间质纤维化的关系。方法采用单侧输尿管结扎致肾间质纤动物模型,将30只Wistar雄性大鼠,随机平均分为假手术组、模型组和治疗组,术后14 d,测肾功能,免疫组织化学、原位杂交法,测Ⅳ型胶原蛋白、肌动蛋白α(α-SMA)、组织蛋白酶抑制剂1/基质金属蛋白酶9 mRNA的表达。结果模型组、治疗组血尿素氮、血肌酐,Ⅳ型胶原蛋白、α-SMA、组织蛋白酶抑制剂1水平高于假手术组(P<0.05);而治疗组与模型组相比MMP-9水平显著降低(P<0.05)。结论 IGF-1参与梗阻性肾病的病理生理过程,导致间质纤维化和肾功能损害。 |
| 关 键 词： | 胰岛素样生长因子1 肾间质纤维化 金属蛋白酶组织抑制物1 基质金属蛋白酶9 |
Effects of IGF-1 on Renal in Terstitium Fibrosis in Rats |
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| ZHANG Hong , WANG Bing-zeng , ZHANG Xu-ya , MENG Fan-wei. Effects of IGF-1 on Renal in Terstitium Fibrosis in Rats[J]. Medical Recapitulate, 2013, 19(5): 917-918,921 | |
| Authors: | ZHANG Hong WANG Bing-zeng ZHANG Xu-ya MENG Fan-wei |
| Affiliation: | .(Department of Pediatrics,the First Affiliated Hospital of Henan University,Kaifeng 475001,China) |
| Abstract: | Objective To investigate the effects and mechanisms of IGF-1 in rats with unilatreal ureteral obstruction(UUO).Methods 30 UUO Wistar male rats were randomly divided into pseudo-operation group,model group and treatment group.14 days after the operation,the renal function was tested.The expression of col-Ⅳ,α-SMA,TIMP-1/MMP-9mRNA in kidneys were observed by immunohistochemistry and in situ hybridization.Results BUN,Ser col-Ⅳ,α-SMA,TIMP-1 mRNA of the model group and treatment group were higher than the pseudo-operation group(P<0.05);MMP-9 of the treatment group was significantly lower than the model group(P<0.05).Conclusion IGF-1 participates in the pathophysiological process,resulting in the renal interstitial fibrosis and renal function impairment. |
| Keywords: | Insulin-like growth factor-1 Renal tubulointerstitial fibrosis Tissue inhibitor of metalloproteinase-1 Matrix metalloproteinases-9 |
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