雌激素对大鼠骨髓基质细胞增殖和分化的影响

目的：研究雌激素对大鼠骨髓基质细胞增殖和分化的影响，探讨其对成骨的影响。方法：体外培养4周龄雌性大鼠骨髓基质细胞，以不同浓度雌激素作用于成骨细胞，用噻唑蓝法测定细胞增殖情况；对硝基酚磷酸酯法测定细胞碱性磷酸酶活性；检测培养液中羟脯氨酸含量，了解细胞Ⅰ型胶原的分泌情况；通过矿化结节计数反映细胞的矿化能力。结果：雌激素促进成骨细胞增殖，增加碱性磷酸酶活性；提高培养液中羟脯氨酸含量，峰值浓度为10^-7mol／1，与对照组比较有统计学意义（P〈0．05）；雌激素显著提高细胞的矿化能力，10^-7mol／L浓度作用最显著（＋45．4％，P〈0．05）。结论：雌激素促进成骨细胞增殖和分化，提高其矿化能力，从而刺激骨形成。

Effect of estrogen on proliferation and differentiation of rat bone marrow stromal cells

Objective:To study the effect of estrogen on proliferation and differentiation of rat bone marrow stromal cells and to explore the mechanism of stimulation of bone formation by estrogen. Methods:Bone marrow stromal cells were isolated from four-week-old female Sprague-Dawley rats and cultured in DMEM medium to be induced into osteoblasts. They were cultured in medium with estrogen at different concentrations. The prolifereation, ALP activity and type-I pro-collagen of osteoblasts were examined by MTT, PNPP and assaying the level of hydroxyproline. The capacity of mineralization were measured by counting bone nodules. Results:The cell quantity, the alkaline phosphatase activity and the level of hydroxyproline of osteoblasts were increased , and the number of nodules was significantly higher. The peak value of all these stimulating effects on osteoblasts was in 10-7mol/L group. Conclusion:Estrogen can increase proliferation, differentiation and the capability of mineralization of osteoblast and this may contribute to bone formation.