膀胱癌PCNA蛋白表达及其与复发的关系

[目的]探讨增殖细胞核抗原（proliferating cell nuclear antigen,PCNA）蛋白在膀胱癌中的表达情况,并分析其与临床病理特征及复发的关系。[方法]148例膀胱移行细胞癌患者标本,25例癌旁正常组织标本作为对照,采用免疫组化SP法检测PCNA蛋白的表达。[结果]癌旁正常组织中PCNA蛋白的阳性表达水平明显低于肿瘤组织（16.0%vs50.7%,P〈0.001）。PCNA蛋白的表达与膀胱癌的分级、分期、复发有关（P〈0.05）,与患者的年龄、性别、肿瘤数目及肿瘤大小无关（P〉0.05）。本组104例浅表性膀胱癌（Ta～T1）随访2～95个月,其中复发64例,复发组与未复发组PCNA蛋白的阳性表达率分别为56.3%和15.0%（P=0.001）。[结论]PCNA蛋白在膀胱癌中高表达,其高表达提示预后不良。

The Expression of PCNA Protein and its Relationship with Recurrence of Bladder Cancer

[Purpose] To investigate the expression of proliferating cell nuclear antigen （PCNA） in bladder cancer,and its relationship with clinicopathologic factors and recurrence of bladder cancer. [Methods] Tissue samples from 148 cases with bladder transitional cell carcinoma and from 25 cases with normal bladder as control group were studied. The expression of PCNA protein was detected by SP immunohistochemistry. [Results] The positive rate of PCNA protein in bladder cancer was significantly higher than that in normal bladder tissue（16.0% vs 50.7%,P0.001）. The positive rate of PCNA protein in bladder cancer was related to grade,clinical stage and recurrence,not related to age,sex ,tumor number and size（P0.05）.One hundred and four patients with superficial bladder cancer （Ta～T1） was followed up for 2～95 months,and 64 cases was recurrence.The positive rate of PCNA protein in recurrence patients and non-recurrence patients was 56.3% and 15.0% respectively （P=0.001）. [Conclusion] PCNA protein was over expression in bladder cancer,it may relate to poor prognosis.