食管癌细胞MUC1过表达对5-氟尿嘧啶及顺铂化疗效果的影响

目的探讨食管癌细胞MUC1过表达对5-氟尿嘧啶及顺铂化疗效果的影响。方法构建MUC1过表达及稳定沉默食管癌细胞株，建立食管癌细胞裸鼠移植瘤模型；顺铂（8mg／kg，d1，d7）及5-氟尿嘧啶（20mg／kg，d1～d6）腹腔注射，测量肿瘤体积及裸鼠体重，绘制生长曲线及体重曲线；计算肿瘤抑瘤率。结果顺铂与5-氟尿嘧啶均能抑制MUC1过表达食管癌移植瘤的生长，裸鼠体重及肿瘤体积与对照组比较，差异有统计学意义（P〈0．05），且顺铂的抑制效应更明显（P〈0．05）；在MUC1稳定沉默裸鼠无明显的抑制效应。结论顺铂与5-氟尿嘧啶都能抑制MUC1过表达食管癌移植瘤的生长，顺铂的抑制效应更明显，同时对体重的影响也更明显。

Effect of MUCI Over-expression on Chemotherapy of 5-Fluorouracil and Cisplatin for Esophageal Cancer Cells

Objective To investigate MUC1 over-expression on chemotherapy of 5-fluorouracil and cisplatin for esophageal cancer cells. Methods MUC1 over-expression and stable silencing of MUC1 expression esophageal cancer cell lines were constructed. Xenograft model of esophageal cancer was established in nude mice. Cisplatin （ 8 mg/kg, day 1 and day 7 ） and 5-fluorouracil （20 mg/kg, day 1 to 6 ） were injected intraperitoneally. Tumor volume and body weight of nude mice were measured. Tumor growth curve and body weight curve were drawn, and tumor inhibitory rate was calculated. Results Both cisplatin and 5-fluorouracil suppressed tumor growth of MUC 1 over-expression esophageal cancer nude mice. Body weight and tumor volume of nude mice of cisplatin and 5-fluorouracil groups were significantly smaller than those of the control group （P 〈 0.05 ）, and the inhibitory effects of cisplatin were significantly greater than those of 5-fluorouracil （P 〈 0.05）. There was no significant inhibitory effect in stable silencing of MUC1 expression esophageal cancer nude mice. Conclusion Both cisplatin and paclitaxel can suppress the growth of MUC1 over-expression esophageal cancer, and cisplatin has greater inhibitory effects than 5-fluorouracil in tumor volume and body weight of nude mice.