| 绿藻硫酸多糖UCS2的结构及抗凝血活性研究 |
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| 引用本文： | 张俊艳,杨亚靖,褚晓,何美佳,刘山,毛文君.绿藻硫酸多糖UCS2的结构及抗凝血活性研究[J].中国海洋药物,2022,41(6):57-61. |
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| 作者姓名： | 张俊艳 杨亚靖 褚晓 何美佳 刘山 毛文君 |
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| 作者单位： | 中国海洋大学 海洋药物教育部重点实验室,中国海洋大学 海洋药物教育部重点实验室,中国海洋大学 海洋药物教育部重点实验室,中国海洋大学 海洋药物教育部重点实验室,中国海洋大学 海洋药物教育部重点实验室,中国海洋大学 海洋药物教育部重点实验室 |
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| 基金项目： | 国家自然科学(41476108)；山东省重大科技创新工程专项(2018SDKJ0401-3)资助 |
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| 摘 要： | 目的 对绿藻多糖UCS2的结构特征和抗凝血活性进行研究。方法 通过冷水提取、强阴离子交换色谱和凝胶渗透色谱，从绿藻花石莼中提取分离得到硫酸多糖UCS2；采用高效凝胶渗透色谱（HPGPC）、高效液相色谱、红外光谱和气质联用色谱对多糖UCS2的结构进行表征；通过活化部分凝血活酶时间（APTT）、凝血酶时间、凝血酶原时间对多糖UCS2体外抗凝血活性进行研究。结果 绿藻多糖UCS2分子量为39.55kDa，硫酸根和糖醛酸含量分别为19.74%和18.66%；UCS2主要由鼠李糖组成，含有少量葡糖醛酸和木糖。糖链中含有→3,4)-α-L-Rhap-(1→, →4)-α-L-Rhap-(1→, →4)-β-D-Xylp-(1→和→4)-β-D-GlcAp-(1→，硫酸基位于→4)-α-L-Rhap-(1→的C-3位。多糖UCS2对APTT有显著延长作用，具有较高的抗凝活性。结论 绿藻多糖UCS2是1种抗凝活性的葡萄糖醛酸-木糖-鼠李糖型硫酸多糖。
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| 关 键 词： | 绿藻多糖 结构表征 抗凝血活性 |
| 收稿时间： | 2021/11/29 0:00:00 |
| 修稿时间： | 2022/2/17 0:00:00 |
Structure characterization and anticoagulant activity of the green algal sulfated polysaccharide UCS2 |
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| ZHANG Jun-yan,YANG Ya-jing,CHU Xiao,HE Mei-ji,LIU Shan and MAO Wen-jun.Structure characterization and anticoagulant activity of the green algal sulfated polysaccharide UCS2[J].Chinese Journal of Marine Drugs,2022,41(6):57-61. |
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| Authors: | ZHANG Jun-yan YANG Ya-jing CHU Xiao HE Mei-ji LIU Shan and MAO Wen-jun |
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| Institution: | Key Laboratory of Marine Drugs,Ministry of Education,Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering,School of Medicine and Pharmacy,Ocean University of China,Key Laboratory of Marine Drugs,Ministry of Education,Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering,School of Medicine and Pharmacy,Ocean University of China,Key Laboratory of Marine Drugs,Ministry of Education,Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering,School of Medicine and Pharmacy,Ocean University of China,Key Laboratory of Marine Drugs,Ministry of Education,Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering,School of Medicine and Pharmacy,Ocean University of China,Key Laboratory of Marine Drugs,Ministry of Education,Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering,School of Medicine and Pharmacy,Ocean University of China,Key Laboratory of Marine Drugs,Ministry of Education,Shandong Provincial Key Laboratory of Glycoscience and Glycoengineering,School of Medicine and Pharmacy,Ocean University of China |
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| Abstract: | |
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| Keywords: | green alga polysaccharide structural characterization anticoagulant activity |
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