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| 健脾解毒方介导p38MAPK信号转导下调幽门螺杆菌诱导的胃癌细胞环氧合酶2启动子活性 | |
| 引用本文： | 王炎,刘宁宁,周利红,吴琼,周宁,孙珏,范忠泽,李琦.健脾解毒方介导p38MAPK信号转导下调幽门螺杆菌诱导的胃癌细胞环氧合酶2启动子活性[J].中国实验方剂学杂志,2010,16(14):105-109. |
| 作者姓名： | 王炎 刘宁宁 周利红 吴琼 周宁 孙珏 范忠泽 李琦 |
| 作者单位： | 1. 上海中医药大学附属普陀医院实验中心,上海,200062 2. 上海中医药大学附属普陀医院肿瘤科,上海,200062 3. 上海中医药大学附属普陀医院实验中心,上海,200062;上海中医药大学附属普陀医院肿瘤科,上海,200062 |
| 基金项目： | 国家自然科学基金项目，上海市普陀区科委科技创新重大项目，上海市重点学科资助项目，上海市教委青年基金 |
| 摘 要： | 目的:探讨幽门螺杆菌(Helicobacter pylori)对人胃癌MKN45细胞环氧合酶2(Cyclooxygenase-2,COX-2)启动子荧光素酶报告基因重组质粒(pGL3-Basic-COX-2-promoter)活性的影响和健脾解毒方对其的调控作用及可能的机制。方法:将构建的pGL3-Basic-COX-2-promoter转染MKN45细胞,观察H.pylori对其活性的影响。运用p38MAPK特异性抑制剂SB203580阻断p38MAPK信号通路,观察H.pylori对MKN45细胞COX-2启动子活性的影响。Western blot法检测健脾解毒方对H.pylori感染的MKN45细胞p38MAPK信号通路及其下游激活转录因子-2(ATF-2)表达的影响。结果:H.pylori可增加COX-2启动子活性;抑制p38MAPK信号通路后,COX-2启动子活性明显下调;健脾解毒方能够抑制H.pylori诱导的p38MAPK及ATF-)的活性。结论:H.pylori通过p38MAPK信号通路上调胃癌细胞COX-2启动子活性;健脾解毒方通过调控p38MAPK/ATF-2信号通路,抑制COX-2启动子活性,是其防治H.pylori诱发胃癌的机制之一。 |
| 关 键 词： | 胃癌 幽门螺杆菌 环氧合酶2 启动子 健脾解毒方 p38促分裂素原活化蛋白激酶 激活转录因子-2 |
| 收稿时间： | 3/5/2010 12:00:00 AM |
Jianpi Jiedu Recipe Intervenes Signal Transduction of p38 Mitogen-activated Protein kinases and Inhibits Activity of Cyclooxygenase-2 Promoter Induced by Helicobacter pylori in Gastric Cancer Cell MKN45 |
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| WANG Yan,LIU Ning-ning,ZHOU Li-hong,WU Qiong,ZHOU Ning,SUN Yu,FAN Zhong-ze and LI Qi.Jianpi Jiedu Recipe Intervenes Signal Transduction of p38 Mitogen-activated Protein kinases and Inhibits Activity of Cyclooxygenase-2 Promoter Induced by Helicobacter pylori in Gastric Cancer Cell MKN45[J].China Journal of Experimental Traditional Medical Formulae,2010,16(14):105-109. | |
| Authors: | WANG Yan LIU Ning-ning ZHOU Li-hong WU Qiong ZHOU Ning SUN Yu FAN Zhong-ze and LI Qi |
| Institution: | Laboratory Center, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200062,China;Department of Oncology, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China;Laboratory Center, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200062,China;Laboratory Center, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200062,China;Laboratory Center, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200062,China;Department of Oncology, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China;Department of Oncology, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China;Laboratory Center, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200062,China;Department of Oncology, Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine, Shanghai 200062, China |
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