P16蛋白的表达在子宫颈上皮内瘤变中的诊断价值

背景与目的： 探讨和评价P16的表达在子宫颈上皮内瘤变中的诊断价值。 材料与方法： 用免疫组化S-P法对40例慢性宫颈炎伴腺体鳞化、40例宫颈尖锐湿疣、30例子宫颈上皮内瘤变Ⅰ型(CINⅠ)、25例CINⅡ、20例CINⅢ和30例宫颈浸润性鳞癌中的P16蛋白的表达进行检测。 结果： 40例慢性宫颈炎伴腺体鳞化中38例不着色，40例宫颈尖锐湿疣中34例呈局灶型着色，CIN及宫颈浸润性鳞癌中，除3例 CINⅠ外，均为弥漫型着色。 结论： P16的着色可用于CIN与慢性宫颈炎伴腺体鳞化、尖锐湿疣之间的鉴别。在检查破碎小块组织和薄层上皮CIN病变时P16的表达可起辅助作用。

Diagnostic Significance of P16 Protein Expression in Cervical Intraepithelial Neoplasia

BACKGROUND ＆ AIM: To investigate and evaluate the usefulness of P16 expression in the diagnosis of cervical intraepithelial neoplasia. MATERIAL AND METHODS: Samples from 40 patients with chronic cervicitis associated with squamous metaplasia, 40 with condyloma acuminata, 30 with CINⅠ, 25 with CINⅡ, 20 with CINⅢ and 30 patients with invasive cervical squamous carcinoma were immunohistochemically stained. RESULTS： 38 of 40 chronic cervicitis with squamous metaplasia did not stain, 34 of 40 condyloma acuminata stained focally, all CIN and invasive squamous carcinoma cases, except for 3 CINⅠcases, stained diffusely. CONCLUSION： The staining pattern of P16 was useful to differentiate CIN with chronic cervicitis with squamous metaplasia and cervical condyloma acuminatum. The expression of P16 could be helpful to diagnose CIN in small fragmented tissues or thin epithelium.