三氧化二砷联合热疗抗人大肠癌细胞的实验研究

目的：探讨三氧化二砷（As2O3）注射液联合热疗对人大肠癌细胞LoVo生长的影响及其作用机制。方法：经As2O3注射液联合热疗处理LoVo细胞后采用MTY法测定细胞的增殖抑制效应；应用透射电镜、AO／EB荧光染色法、流式细胞术观察细胞凋亡和细胞周期变化。结果：与As2O3单药或单用热疗相比，As2O3与热疗联合应用后可显著增强抑制LoVo细胞增殖和诱导细胞凋亡作用，并使细胞S期比例明显降低，G0／G1期明显升高。结论：As2O3联合热疗具有协同抗大肠癌作用，其机制可能与联合应用后增强诱导大肠癌细胞凋亡、细胞周期特异性治疗与细胞周期调控剂联合增效有关。

The effect of arsenic trioxide (As2O3) combined with hyperthermia on coloreotal cancer cells

Objective:To study the effect of arsenic trioxide (As2O3) combined with hyperthermia on the growth of colorectal cancer cell line LoVo and to discuss its possible mechanism. Methods:In every group, the percentage of survival was calculated with MTT assay, the apoptosis and the cell cycle were observed by transmission electron microscopy, acridine orange(AO)/ethidiumbromide(EB) fluresent staining and flow cytometry.Results:As2O3 combined with hyperthermia could obviously inhibit cell proliferation and induce apoptosis as compared with As2O3 or hyperthermia. A pronounced increase in the S-phase was observed exposed to combination, accompanied by a marked decline in the G0/G1-phase. Conclusion:As2O3 in combination with hyperthermia can produce synergistic killing effect on LoVo cell.This may be related with the increase of apoptosis and cell cycle specific killing combined with cell cycle regulator.