抗心肌肌球蛋白抗体在慢性病毒性心肌炎小鼠中的表达及卡托普利、氧化苦参碱的干预效果

目的 观察抗心肌肌球蛋白抗体(AMA)在慢性病毒性心肌炎(VMC)小鼠中的表达,比较卡托普利和氧化苦参碱的干预效果.方法 以柯萨奇病毒B_3株(CVB_3)重复增量感染(第0、14、28天,剂量分别为0.20、0.25、0.30mL)BALB/c小鼠建立慢性VMC(n=60)模型,正常对照组(n=8)同期腹腔注射等体积生理盐水.42d将慢性VMC模型组存活小鼠随机分为慢性VMC对照组(n=8)、卡托普利干预组(n=8)及氧化苦参碱干预组(n=8),干预28d后,即实验第70天杀死各组小鼠.称取小鼠心脏质量,计算心脏与体质量比值;分别以苏木素-伊红(HE)、苦味酸天狼猩红行心肌组织及心肌胶原组织特异性染色,运用图像分析软件计算心肌组织中胶原容积积分(CVF,%);酶联免疫法(ELISA)检测实验小鼠血清中AMA的表达,以光密度值(OD值)>(x-)+2s为阳性组,<(x-)+2s为阴性组.结果 慢性VMC组小鼠在实验第70天心脏与体质量比值、CVF及血清中AMA OD值均较正常对照组增高(P均<0.01).卡托普利及氧化苦参碱干预后心脏与体质量比值、CVF较慢性VMC组降低(P均<0.01),血清中AMA OD值较慢性VMC组降低(P<0.05).AMA阳性组心脏与体质量比值、CVF及OD值均较阴性组增高(P均<0.01).结论 慢性VMC小鼠血清中AMA表达增高,卡托普利及氧化苦参碱可下调AMA的表达,抑制慢性VMC小鼠的心肌重构.

Interventional effects of Captopril and Oxymatrine on anti-cardiac myosin antibody expression in mice with chronic viral myocarditis

Objective To observe the expression of anti-cardiac myosin antibody(AMA) in mice with chronic viral myocarditis (CVMC),and to compare the interventional effects of Captopril and Oxymatrine. Methods Balb/c mice (n=60) were infect with CVB3 at days 0,14,28 to establish CVMC models. The volumes of CVB3 were 0.20,0.25 and 0.30 mL respectively. A normal control group (n=8) received an equal-volume normal saline without CVB3 at the same time. The mice that survived in the CVMC model group were randomly divided in...