In Vitro Uptake of Gentamicin by Rat Renal Cortical Tissue |
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| Authors: | Chen H. Hsu Theodore W. Kurtz John M. Weller |
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| Affiliation: | 1.Nephrology Division, Department of Internal Medicine, The University of Michigan, Ann Arbor, Michigan 48109 |
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| Abstract: | The mechanism of gentamicin uptake in vitro by renal cortical slices of rat kidney was investigated. The cortical-slice-uptake ratio of gentamicin concentration in 1.0 g of tissue water to that of 1.0 ml of incubation medium (SW/M) was 1.44 ± 0.04. The uptake of gentamicin was inhibited by 2 × 10−5 M dinitrophenol (SW/M = 1.03 ± 0.04) and by anoxia (SW/M = 1.01 ± 0.04). The results indicate that aerobic phosphorylation is required to transport gentamicin into the cells. The uptake of p-aminohippurate and tetraethylammonium chloride by renal cortical slices was not affected by gentamicin. |
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