危重监护病房铜绿假单胞菌耐药性及脉冲场凝胶电泳分析

目的　了解我院危重监护病房 (简称 ICU)铜绿假单胞菌的耐药性。建立一种快速、准确、可靠、重复性好的基因分型方法来研究 ICU铜绿假单胞菌的分子流行病学。方法　采用全自动鉴定仪 ( Walkaway-4 0 DADE Microscan Inc)鉴定铜绿假单胞菌 ,并根据半定量微量肉汤系统测定 7种常用抗生素对细菌的最低抑菌浓度 ,确定其耐药情况。用脉冲场凝胶电泳 (简称 PFGE)对限制性酶切片段分离。通过对每一株菌染色体DNA指纹图谱的比较分析其克隆构成 ,了解其流行状况。结果　 2 4株菌对 7种抗生素的耐药性由高到低依次为 :环丙沙星 ( 87.5 % ) ,庆大霉素 ( 75 % ) ,亚胺培南 ( 6 6 .7% ) ,头孢曲松 ( 6 6 .7% ) ,哌拉西林 ( 2 9.2 % ) ,头孢他啶 ( 2 0 .8% ) ,阿米卡星 ( 12 .5 % )。8株菌经 PFGE分析初步证实其中 4株菌具有明显相关性。结论　铜绿假单胞菌对常用抗菌药物的耐药率高 ,多重耐药情况严重。 PFGE具有分辨力高、重复性好的特点 ,是研究 ICU铜绿假单胞菌分子流行病学较好的基因分型方法

Antibiotic resistance and genotyping of Pseudomonas aeruginosa isolated from ICU by pulsed-field gel electrophoresis

Objective To analyze antibiotic resistance pattern and establish an accurate and rapid genotyping method to study the molecular epidemiology of Pseudomonas aeruginosa isolated from ICU. Methods MICs of 24 Pseudomonas aeruginosa isolates against 7 antibiotics were detected with semi quantitative method. Among them, 8 isolates were chosen for genotyping by pulsed field gel eletrophoresis (PFGE). Result Four P.aeruginosa strains in ICU were epidemiological related with no statistic difference. Conclusion Multi antibiotic resistance of Pseudomonas aeruginosa isolated from ICU was critical and PFGE is a better genotyping method to study molecular epidemiology and analytic homology.