薄荷总黄酮的纯化工艺优选

目的:优选聚酰胺纯化薄荷总黄酮的工艺条件。方法:以总黄酮含量和吸附-洗脱率为指标,采用单因素试验考察5种不同类型的纯化材料对总黄酮的吸附能力,确定纯化材料,并对其纯化工艺中吸附条件、除杂条件和洗脱条件进行优化。结果:优化工艺条件为上样液质量浓度0.1 g.mL-1,上样量为生药量与聚酰胺体积比1∶6,吸附流速2 BV.h-1,柱床径高比1∶9,待吸附结束后,用3 BV 10%乙醇洗脱除杂,除杂流速4 BV.h-1,再用3 BV 90%乙醇以8 BV.h-1流速洗脱,经聚酰胺(30～60目)纯化后,薄荷总黄酮纯度可达50%。结论:优选的纯化工艺分离效果良好、操作简单、重复性好,可用于分离富集薄荷总黄酮。

Optimization of Purification Technology for Total Flavonoids from Mentha haplocalyx

Objective: To optimize polyamide purification technology of total flavonoids from Mentha haplocalyx. Method: With the content and transfer rate of total flavonoids as indexes, adsorption capacity of 5 different kinds of purification materials for total flavonoids was investigated by single factor test, in order to determine optimum purification material, and adsorption, impurity removing and eluting conditions in purification technology were optimized. Result: Optimum purification conditions were as follows:The concentration of sample solution 0.1 g·mL^-1, diameter-height ratio of column 1:9, adsorption rate 2 BV·h^-1, proportion between the total amount of solution and resin column 1:6, 3 BV of 10% ethanol to wipe off impurity with flow rate of 4 BV·h^-1, then 3 BV of 90% ethanol to elute with 8 BV·h^-1.After purified by polyamide(30-60 mesh), purity of total flavonoids was up to 50%. Conclusion: This optimized purification technology was simple and feasible with good reproducibility, it could be used for the separation and the enrichment of total flavonoids from M.haplocalyx.