正交试验优选山豆根有效部位的提取工艺

目的:优选山豆根有效部位的提取工艺。方法:采用L9(34)正交试验法,以总生物碱含量为指标,选取乙醇体积分数、加醇量、提取时间及提取次数为考察因素,优选山豆根中总生物碱的提取工艺;以总多糖含量为指标,选取加水倍量、提取时间及提取次数为考察因素,优选山豆根中总多糖的提取工艺。结果:总生物碱最佳提取工艺为加6倍量70%乙醇提取2次,每次1 h;总多糖最佳提取工艺为加6倍量水提取3次,每次1 h。结论:优选的提取工艺操作简单、稳定可行,适合于产业化生产,具有良好的应用前景。

Optimization of Extraction Technology for Effective Parts from Sophora tonkinensis by Orthogonal Test

Objective: To optimize extraction technology of effective parts from Sophora tonkinensis. Method: Taking the content of total alkaloids as index, with extraction time, extraction times, ethanol concentration and amount as factors, extraction technology of total alkaloids from S.tonkinensis was optimized by L₉(3⁴) orthogonal test;With the content of total polysaccharides as index, taking the amount of water, extraction time and times as factors, extraction technology of total polysaccharides was optimized by orthogonal test. Result: Optimal extraction technology of total alkaloids was as following:extracted 2 times with 6 times the amount of 70% ethanol, 1 h per time;Optimum extraction technology of total polysaccharides was as following:extracted 3 times with 6 times the amount of water, 1.0 h each time. Conclusion: This optimized extraction technology was stable and feasible, it was suitable for industrial production with a good prospect.