母血中胎儿DNA与子痫前期关系的研究

目的:探讨孕妇外周血中胎儿DNA的浓度水平与子痫前期之间的关系。方法:留取第一次常规产前检查的472例孕妇的外周血并随访。设置阴性对照后,采用实时PCR方法定量检测怀男性单胎并发展为子痫前期的17例孕妇及34例怀男性单胎正常孕妇的血浆胎儿DNA的SRY基因浓度。结果:发展为子痫前期患者的外周血浆胎儿DNA浓度明显高于正常孕妇,中位数分别为165.41copies/ml和48.75copies/ml,有显著的统计学差异(P<0.001)。结论:发展为子痫前期患者外周血中的胎儿DNA明显增高,而浓度水平与子痫前期的严重程度似乎无关。

Significance of increased fetal DNA concentration in maternal serum of preeclampsia patients

Objective:To study the relationship between the abnormal increase of circulating fetal DNA concentration and preeclampsia.Methods:Maternal blood were collected from 472 pregnant women attending for the first antenatal visit and the patients were followed up.Real-time polymerase chain reaction(PCR) using TaqMan primers and probes directed against SRY gene sequences were used to quantify fetal DNA in the maternal circulation who subsequently developed preeclampsia ,and that of the normal control group were also quantified.Result:The median of fetal DNA level of two group was 165.41copies/ml and 48.75copies/ml,respectively.The difference were significant(P<0.001).Conclusion:Fetal DNA was increased in the maternal circulation in pregnancy women who subsequently develop preeclampsia,and there appears to be no relationship between the quantity of fetal DNA and the risk of developing preeclampsia.