广西壮族人群稀有血型筛选

了解广西壮族人群中Ena-、Lub-、Ge-、Jk(a-b-)红细胞血型的频率与分布情况,为建立符合本地区血型抗原分布格局合理的谱细胞进行临床输血前检查提供依据。采用立即离心试管法筛选Mur+、H-;用试管法间接抗球蛋白试验或96孔微量板法直接离心试验筛选DCE/DCE、DCE/dCE、dCE/dCE表型;96孔微量板法直接离心试验和间接抗球蛋白试验筛选Lub-、Ena-、Ge-、Wrb-和i;2 mol/L尿素溶血试验筛选JK(a-b-)表型。在4 527名壮族人群中筛选出Lub-57例、Jk(a-b-)2例;对其中2 825例壮族筛选出Mur+319例,没有发现Ena-、Ge-、Wrb-、类孟买血型、成人i及DCE/DCE、DCE/dCE、dCE/dCE表型。结果显示,广西壮族人群Jk(a-b-)稀有表型频率(0.044%)高于欧洲白人、我国上海和广东番禺地区人群;Lub-表型频率1.26%;Mur+频率(11.29%)高于上海及广东番禺地区,但明显低于云南怒族人群。

Screening of the rare blood group in Zhuang population of Guangxi province

To discover the frequency and distribution of the blood group of red blood cells Ena-、 Lub-、 Ge-、 Jk(a-b-) in Zhuang population,provide the evidence of checking rational spectrum cells which are established to meet local distribution pattern of blood group antigens before clinical transfusion.Mur+,H-were screened by immediately centrifugal tube method;DCE/DCE、 DCE/dCE、 dCE/dCE phenotype were screened by indirect antiglobulin test or 96 microplate directly centrifugal method;Lub-,Ena-,Ge-,Wrb-and i were screened by 96 microplate directly centrifugal method and indirect antiglobulin test;JK(a-b-) phenotype was screened by 2 mol/L urea hemolysis test.57 Lub-and 2 Jk(a-b-) were screened in 4 527 Zhuang population;319 Mur+ were screened in 2 825 which was a part of 4 527 Zhuang population.None of Ena-,Ge-,Wrb-,Bombay blood group,adult I,CDE/CDE and CdE/CdE and dCE/dCE phenotype was found.This result demonstrates that the rare phenotype frequency(0.044%) of Jk(a-b-) in Guangxi Zhuang population is higher than in European Caucasian,Chinese Shanghai and Guangdong Panyu population.The phenotype frequency of Lub-is 1.26%,the frequency of Mu+(11.29%) is higher than in Shanghai and Guangdong Panyu,but is apparently lower than in Yunnan Nu population.