| 山羊乳牙牙髓细胞复合多孔磷酸钙骨水泥异位成骨研究 |
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| 引用本文： | Zhao W,Hao YM,Zou DR,Zhang XL,Lu JY,Hua L,Cao CH. 山羊乳牙牙髓细胞复合多孔磷酸钙骨水泥异位成骨研究[J]. 上海口腔医学, 2011, 20(5): 464-469 |
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| 作者姓名： | Zhao W Hao YM Zou DR Zhang XL Lu JY Hua L Cao CH |
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| 作者单位： | 1. 上海交通大学附属第六人民医院,口腔科,上海,200233
2. 上海交通大学医学院附属第九人民医院,上海市口腔医学重点实验室,上海,200011 |
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| 基金项目： | 上海市科学技术委员会资助项目(09JC1411700); 上海市口腔基础医学开发科研合作项目(S30206-KF09); 上海交通大学医学院基金项目(09XJ21030)~~ |
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| 摘 要： | 目的:观察山羊乳牙牙髓细胞(SGDs)与多孔磷酸钙骨水泥之间的生物相容性及其异位成骨能力。方法:流式细胞仪检测第1代SGDs的STRO-1表达情况;体外培养的第4代SGDs进行成骨诱导7d后,与多孔磷酸钙骨水泥复合培养,扫描电镜下观察SGDs黏附和生长情况;细胞材料复合物植入裸鼠皮下8周观察异位成骨能力。结果:SGDs与多孔磷酸钙骨水泥复合培养3d后,细胞与材料表面贴合,形态正常,可见细胞伸出伪足,分泌胞外基质;裸鼠体内植入8周后,HE染色显示骨样组织形成,免疫组化OC呈阳性表达。结论:SGDs可以向成骨细胞分化,并具有诱导成骨能力,与多孔磷酸钙骨水泥结合,可形成骨样组织。
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| 关 键 词： | 山羊乳牙牙髓细胞 多孔磷酸钙骨水泥 组织工程 异位植入 成骨 |
Experimental study on stem cells from goat deciduous teeth compounded with porous calcium phosphate cement for ectopic osteogenesis |
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| Zhao Wei,Hao Yong-ming,Zou De-rong,Zhang Xiu-li,Lu Jia-yu,Hua Li,Cao Cun-hua. Experimental study on stem cells from goat deciduous teeth compounded with porous calcium phosphate cement for ectopic osteogenesis[J]. Shanghai journal of stomatology, 2011, 20(5): 464-469 |
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| Authors: | Zhao Wei Hao Yong-ming Zou De-rong Zhang Xiu-li Lu Jia-yu Hua Li Cao Cun-hua |
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| Affiliation: | ZHAO Wei1,HAO Yong-ming1,ZOU De-rong1,ZHANG Xiu-li2,LU Jia-yu1,HUA Li1,CAO Cun-hua1.(1.Department of Stomatology,Sixth People's Hospital,Shanghai Jiao Tong University.Shanghai 200233,2.Oral Bioengineering Lab/Oral Tissue Engineering Lab,Shanghai Research Institute of Stomatology,Ninth People's Hospital,Shanghai Jiao Tong University School of Medicine.Shanghai 200011,China) |
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| Abstract: | PURPOSE: To observe the biocompatibility and ectopic bone-like tissue formation of stem cells from goat deciduous teeth(SGDs) with porous calcium phosphate cement(pCPC).METHODS: The expression of STRO-1 on SGDs was measured with flow cytometry(FCM);the 4th passage SGDs were cultured in induced-mineralization medium in vitro for 7 days.Combined with pCPC,the adhesion and growth of the compounds were observed with scanning electron microscopy(SEM);the ectopic bone-like tissue formation was observed 8 weeks af... |
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| Keywords: | Stem cells from goat deciduous teeth Porous calcium phosphate cement Tissue engineering Ectopic implantation Osteogenesis |
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