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| 高效液相色谱法测定肝安胶囊中水飞蓟宾的含量 | |
| 引用本文： | 王慧,路克军,张洁瑾,栾晓琳,周燕妮,张国庆. 高效液相色谱法测定肝安胶囊中水飞蓟宾的含量[J]. 药学实践杂志, 2013, 31(6): 459-461 |
| 作者姓名： | 王慧 路克军 张洁瑾 栾晓琳 周燕妮 张国庆 |
| 作者单位： | 第二军医大学附属东方肝胆外科医院药材科,上海200438 |
| 摘 要： | 目的建立高效液相色谱法测定肝安胶囊中水飞蓟宾的含量。方法使用Agilent Zorbax SB—C18柱（4．6mm×250mm，5μm），流动相：1％冰醋酸-甲醇=50：50，流速：1．0ml／min，柱温：30℃，检测波长：287nm，测定10批肝安胶囊中水飞蓟宾的含量。结果水飞蓟宾在10．28～123．36μg／ml（r=1）浓度范围内线性关系良好，回收率为97．00％（RSD=0．68％）。10批肝安胶囊中水飞蓟宾含量为11．11～16．65mg／粒。结论该方法简便、准确、重复性好，可以用于肝安胶囊的质量控制。 |
| 关 键 词： | 高效液相色谱法 水飞蓟宾 肝安胶囊 含量测定 |
| 收稿时间： | 2012-09-26 |
| 修稿时间： | 2013-03-26 |
Determination of silybin contents in Gan'an capsule by HPLC |
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| WANG Hui,LU Ke-jun,ZHANG Jie-jin,LUAN Xiao-lin,ZHOU Yan-ni and ZHANG Guo-qing. Determination of silybin contents in Gan'an capsule by HPLC[J]. The Journal of Pharmaceutical Practice, 2013, 31(6): 459-461 | |
| Authors: | WANG Hui LU Ke-jun ZHANG Jie-jin LUAN Xiao-lin ZHOU Yan-ni ZHANG Guo-qing |
| Affiliation: | ( Departement of Pharmacy, Eastern Hepatobilliary Hospital, Second Military Medical University, Shanghai 200438,China) |
| Abstract: | Objective To establish a HPLC method for determination of silybin contents in Gan＇an capsule. Methods Angilent Zorbax SB C18（4.6 mm ×250 mm,5 μm） column was used with 1% acetic acid ： methanol =50 ： 50 as the mobile phase. The flow rate was 1.0 ml/min,and the temperature was at 30℃. The detection wavelength was 287 nm . Determination of silybin contents in Gan＇an capsule of 10 batches of preparations. Results The silybin calibration curve was at the range of 10.28 - 123.36 μg/ml（r = 1 ） with average recovery of 97.00% （ RSD = 0.68% ）. 10 batches of preparations silybin contents in Gan＇an capsule was 11.11 - 16.65 mg/granule. Conclusion The method was simple accurate and reproducible which could be used for the quality control of Gan＇an capsule. |
| Keywords: | HPLC silybin Gan＇an capsule determination |
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