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| 孤儿核受体RORC在脓毒症大鼠肺泡巨噬细胞的表达及调控机制 | |
| 引用本文： | 熊燚,何志江,王兴勇.孤儿核受体RORC在脓毒症大鼠肺泡巨噬细胞的表达及调控机制[J].临床儿科杂志,2010,28(1). |
| 作者姓名： | 熊燚 何志江 王兴勇 |
| 作者单位： | 1. 遵义市妇女儿童医院,贵州遵义,563000 2. 深圳市保安区妇幼保健院,广东深圳,518133 3. 重庆医科大学附属儿童医院,重庆,400014 |
| 摘 要： | 目的观察脓毒症肺损伤大鼠孤儿核受体RORC基因表达及血清IL-17水平,探讨Th17细胞在肺损伤中的作用及可能的调控机制。方法24只SD雄性大鼠随机分为对照组、模型组,吡咯烷二硫代氨基甲酸盐(pyrrolidine dithiocarbamates,PDTC)预防组。静脉注射脂多糖(LPS,6mg/kg)复制急性肺损伤(ALI)的动物模型,在注射后12h处死。PDTC预防组在注射LPS前30min予以PDTC(120mg/kg)腹腔注射。测定肺湿/干重比(W/D),石蜡包埋切片经苏木素-伊红(HE)染色行肺组织病理评分,免疫组织化学法检测肺泡巨噬细胞(alveolar macrophages,AM)核因子-κBP65(nuclear factor-κBP65,NF-κBP65)表达,ELISA法检测血清IL-17水平,实时荧光定量PCR法检测AM孤儿核受体RORC基因表达。结果与对照组相比,模型组肺干/湿比、肺组织病理评分、AM的NF-κBP65表达、血清IL-17水平、AM孤儿核受体RORC基因表达增加(P<0.05);与模型组相比,PDTC预防组上述改变得以逆转。结论Th17细胞可能参与了肺损伤的病理过程... |
| 关 键 词： | 肺损伤 吡咯烷二硫代氨基甲酸盐 孤儿核受体 核因子-κB IL-17 |
Expression of orphan nuclear receptor RORC in rat alveolar macrophages during sepsis and regulation mechanism |
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| XIONG Yi,HE Zhi-jiang,WANG Xing-yong.Expression of orphan nuclear receptor RORC in rat alveolar macrophages during sepsis and regulation mechanism[J].The Journal of Clinical Pediatrics,2010,28(1). | |
| Authors: | XIONG Yi HE Zhi-jiang WANG Xing-yong |
| Institution: | 1.Woman and Children's Hospital in Zunyi/a>;Zunyi 563000/a>;Guizhou/a>;China/a>;2.Bao'an Maternity & Child Health Hospital/a>;Shenzhen 518133/a>;Gunagdong/a>;3.Children's Hospital/a>;Chongqing University of Medicial Science/a>;Chongqing 400014/a>;China |
| Abstract: | Objective To detect the expression of orphan nuclear receptor RORC gene and the level of serum interleukin-17 in acute lung injury (ALI) rats,and to explore the effects and possible regulation mechanisms of helper T lymphocyte 17 (Th17) in the ALI. Methods Twenty four SD male rats were randomly assigned to three groups,control group,model group,and pyrrolidine dithiocarbamates (PDTC) prevention group. The rats in model group and PDTC prevention group were intravenously injected LPS (6 mg / kg) for inducing ... |
| Keywords: | IL-17 lung injury pyrrolidine dithiocarbamates RORC IL-17 |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |