| 外燥对小鼠气管细胞凋亡与bcl-2基因及bax基因表达的影响 |
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| 引用本文： | 丁建中,龚权,陈刚,李军川,陈庭煊,李侃. 外燥对小鼠气管细胞凋亡与bcl-2基因及bax基因表达的影响[J]. 时珍国医国药, 2007, 18(1): 15-16 |
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| 作者姓名： | 丁建中 龚权 陈刚 李军川 陈庭煊 李侃 |
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| 作者单位： | 1. 长江大学医学院,湖北,荆州,434000
2. 湖北中医学院,湖北,武汉,430061 |
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| 基金项目： | 湖北省自然科学基金资助项目(No.2004ABA185) |
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| 摘 要： | 目的观察外燥对小鼠气管细胞凋亡与bc l-2基因及bax基因表达的影响。方法30只昆明种小鼠随机分为常温常湿组(A组),温燥组(B组),凉燥组(C组),每组10只。经处理后第14天用免疫组化法检测各组气管细胞凋亡率(%)、bc l-2、bax阳性指数(PI,%)、bc l-2/bax累积阳性指数(AI,%)与bc l-2:bax蛋白表达比值。结果3组小鼠气管细胞凋亡率均≤0.3%;B组与C组bc l-2 PI与AI均低于A组(P<0.01),B组、C组气管细胞bc l-2:bax蛋白比值中bax增加。结论外燥致气管细胞bc l-2表达降低、bc l-2:bax蛋白比值改变,bc l-2、bax阳性细胞以气道炎性病灶或纤毛缺损处多见,提示与外燥分子致病机制有关。
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| 关 键 词： | 外燥 bcl-2基因 bax基因 细胞凋亡 |
| 文章编号： | 1008-0805(2007)01-0015-02 |
| 修稿时间： | 2006-04-13 |
Effects on Apoptosis and Expression of Bcl - 2 and Bax Gene in Respiratory Intraepithelium of Mice with Outer- Dry |
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| DING Jian-zhong,GONG Quan,CHEN Gang,LI Jun-chuan,CHEN Ting-xuan,LI Kan. Effects on Apoptosis and Expression of Bcl - 2 and Bax Gene in Respiratory Intraepithelium of Mice with Outer- Dry[J]. Lishizhen Medicine and Materia Medica Research, 2007, 18(1): 15-16 |
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| Authors: | DING Jian-zhong GONG Quan CHEN Gang LI Jun-chuan CHEN Ting-xuan LI Kan |
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| Affiliation: | DING Jian-zhong~1,GONG Quan~ |
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| Abstract: | Objective To investigate apoptosis and expression of bcl-2 gene,bax gene in respiratory intraepithelium of mice with outer-dry.Methods 30 kunming mice were!divided into three groups,including group A(normal mice),group B(warm-dry mice),group C(cool-dry mice),10 mice/group.Expression of bcl-2 gene,bax gene and apoptosis were measured on the 14th day.Results There was no significant difference for apoptosis among the three groups.Bcl-2 PI and AI in group B and C were markedly reduced compared with group A(P<0.01),and bax gene increased in the ratio of bcl2:bax protein.Conclusion Outer-dry can decrease the expression of bcl-2 gene,change the ratio of bcl-2:bax protein. |
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| Keywords: | Outer-Dry Bcl-2 gene Bax gene Apoptosis |
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