扩散峰度成像在大鼠海人酸癫痫模型中的研究

目的：通过大鼠海人酸（KA）癫痫模型实验，探讨扩散峰度成像（DKI）应用于原发性癫痫研究的可行性。方法：将KA癫痫模型组（KA组）及正常对照组（NC组）各20只大鼠，在GE3．0T磁共振仪上行常规MRI、DKl及3D-MPRAGE等多序列扫描，在6个部位的灰质（基底节区、海马、杏仁核海马区、颞叶皮层、顶叶皮层、额叶皮层）和3个部位的白质（外囊区、前联合、胼胝体）内选取双侧对称部位的兴趣区（ROI），手动测量各向异性分数（FA）、平均扩散率（MD）、平均峰度值（MK）并进行统计学分析。结果：在KA和NC组间，FA值（双侧均值）差异有统计学意义的部位为杏仁核海马区（分别为0．20±0．04和0．22±0．02，P=0．0337）、前联合（分别为0．22±0．04和0．28±0．06，P=0．002）及胼胝体区（分别为0．21±0．05和0．26±0．03，P=0．0002）；MD值（双侧均值）差异有统计学意义的部位为海马（分别为0．67±0．16和0．74±0．04，P=0．0275）；MK值（双侧均值）差异有统计学意义的部位为基底节区（分别为0．10±0．26和0．83±0．06，P=0．0098）、海马（分别为0．83±0．15和0．73±0．09，P=0．0499）及胼胝体区（分别为0．93±0．22和0．81±0．07，P=0．036）。结论：扩散峰度成像可用于原发性癫痫的研究，有望成为应用于人类癫痫研究的新的影像学方法。

Study on diffusion kurtosis imaging in kainic acid induced epilepsy of rat

Objective：To study the feasibility of diffusion kurtosis imaging （DKI） in the clinical investigation of primary epilepsy with the experiment of investigating kainic acid （KA） induced epilepsy rats and normal control （NC） rats. Methods：Conventional MRI,DKI and 3D-MPRAGE sequences were used in KA induced rats and NC rats （n= 20/group） with a 3.0T MR scanner. In six areas of gray matters （GM） including cauduate putamen （CPu） ,hippocampus （HC） ,amyg dalohippocampal area （AHi）,temporal cerebral cortex （TCT）, parietal cerebral cortex （PCT） and frontal cerebral cortex （FCT） and three areas of white matters （WM） including external capsule （EC） ,Anterior commissure （AC） and corpus callosum （CC）,regions of interest （ROD were selected in symmetric parts of the left and right side. Fractional anisotropy （FA） ,mean diffusivity （MD）,mean kurtosis （MK） values were measured manually and compared, statistic analysis were performed. Results：Of the KA group and NC group of rats,significant differences of FA values （mean FA± SD） were existed in AHi （0.20＋0.04,0.22±0.02 respectively,P=0. 0337） ,AC （0.22±0.04,0.28±0.06 respectively,P=0. 002） and CC area （0.21±0.05,0. 26 ±0.03 respectively, P = 0. 0002）. Significant difference of MD values （mean MD± SD） was existed in HC （0.67±0.16,0.74±0.04 respectively,P=0. 0275）. Significant differences of MK values （mean MK±SD） were existed in CPu （0.10±0.26,0.83±-.06 respectively,P=0. 0098）, HC （0.83±0. 15,0.73±0.09 respectively,P= 0. 0499） and CC areas （0.93±0.22,0.81±0.07 respectively, P= 0. 0360）. Conclusion： The present study demonstrated the successful application of DKI through animal experiment, which propose that DKI sequence could be used as a new method for the research of epilepsy in human beings.