| siRNA沉默HLA-A2基因表达对人脐带间充质干细胞诱导成骨的影响 |
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| 引用本文： | 陈奎,秦书俭. siRNA沉默HLA-A2基因表达对人脐带间充质干细胞诱导成骨的影响[J]. 解剖科学进展, 2010, 16(5) |
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| 作者姓名： | 陈奎 秦书俭 |
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| 作者单位： | 辽宁医学院,人体解剖学教研室,辽宁,锦州,121001 |
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| 摘 要： | 目的探讨RNAi技术下调人脐带间充质干细胞(human umbilical cord mesenchymal stem cells,hUCMSCs)HLA-A2基因表达后,对HLA-A2基因诱导成骨的影响。方法利用HLA-A2靶向小分子干扰RNA(small interfering RNA,siRNA)转染hUCMSCs,实验组为转染的细胞,对照组为未转染细胞。免疫细胞化学染色、Western Blot法检测两组细胞HLA-A2的表达;用诱导剂(0.1μmol/L地塞米松、10mmol/Lβ-甘油磷酸钠、50mg/L维生素C)行成骨诱导分化,茜素红矿化结节染色计数;碱性磷酸酶(ALP)染色以及比色法检测ALP活性。结果免疫细胞化学染色显示,实验组HLA-A2表达为弱阳性,对照组HLA-A2表达为阳性;Western Blot法检测HLA-A2蛋白的表达量对照组明显高于实验组。茜素红矿化结节染色显示:两组细胞均出现红色结节的阳性染色,但无差异(P0.05);两组ALP染色细胞胞浆均呈蓝色阳性反应,ALP活性检测结果显示两组无差异(P0.05)。结论应用RNAi技术下调HLA-A2基因表达后,不影响人脐带间充质干细胞的成骨诱导。
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| 关 键 词： | 人脐带间充质干细胞 HLA-A2基因 RNA干扰 成骨诱导 |
Effect of siRNA silent HLA-A2 gene on the induction of human umbilical cord mesenchymal stem cells into osteogenesis |
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| CHEN Kui,QIN Shu-jian. Effect of siRNA silent HLA-A2 gene on the induction of human umbilical cord mesenchymal stem cells into osteogenesis[J]. Progress of Anatomical Sciences, 2010, 16(5) |
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| Authors: | CHEN Kui QIN Shu-jian |
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| Affiliation: | CHEN Kui; QIN Shu-jian (Department of Anatomy; Liaoning Medical University; Liaoning Jinzhou 121001 China); |
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| Abstract: | Objective To investigate the influence of the downregulation of HLA-A2 gene expression by RNAi on the osteogenic characteristics of umbilical cord mesenchymal stem cells(hUCMSCs).Methods hUCMSCs were transfected by HLA-A2 targeting small interfering RNA(siRNA),and immunohistochemical staining and Western blot were used to detect HLA-A2 expression in transfected and nontransfected hUCMSCs.hUCMSCs were cultured for the differentiation of osteoinduction with DMEM/F12(low sugar) medium(0.1μmol/L dexamethasone,1... |
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| Keywords: | human umbilical cord mesenchymal stem cells HLA-A2 gene RNA interference osteoinduction |
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