人参三七川芎提取物对衰老大鼠免疫器官及行为学的影响

目的:观察人参三七川芎提取物对老龄大鼠免疫器官功能及行为学的影响。方法:选取100只SD大鼠,饲养至18~19月龄,建立自然衰老大鼠模型。按体重水平分层后将成模大鼠随机分为5组,分别为衰老模型组、人参三七川芎中药低、中、高剂量组(2 305.64,1 152.82,576.41 mg·kg-1)和白藜芦醇组(50 mg·kg-1),另设2月龄SD大鼠做为青年对照组。干预3个月后,进行旷场实验,记录大鼠跨越方格总数和总行径距离,检测大鼠血清活性氧族(reactive oxygen species,ROS)含量,胸腺系数和脾脏系数。结果:与青年组大鼠比较,自然衰老组大鼠跨格总数减少(P0.01),总行径距离减少(P0.05),血清ROS含量增高(P0.01),胸腺系数和脾脏指数下降(均P0.01)。与衰老模型组比较,中药各组和白藜芦醇组大鼠的跨格次数和总行径距离均有增加(P0.05);血清活性氧族(ROS)含量降低(P0.05);中药高剂量组和白藜芦醇组可以增高大鼠的胸腺系数和脾脏系数(均P0.01),中药中剂量组可以增高大鼠的胸腺系数(P0.05)。结论:人参三七川芎提取物可以改善自然衰老大鼠精神迟缓状态,增强其空间探索性,同时能够提高免疫器官功能和降低血清活性氧含量。

Effect of Extracts from Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma on Immune Organs and Behavior of Aging Rats

Objective: To observe the effect of extracts from Ginseng Radix et Rhizoma, Notoginseng Radix et Rhizoma and Chuanxiong Rhizoma (GNC)on immune organ function and behavior of aging rats. Method: Totally 100 SD (Sprague Dawley) rats were selected to raise for 18-19 months to establish the model of natural aging rats. According to the horizontal stratification of weight, the model aging rats were randomly divided into 5 groups:aging model group, traditional Chinese medicine low-dose, medium-dose, high-dose groups (2305.64, 1152.82, 576.41 mg·kg^-1) and resveratrol group (50 mg·kg^-1). And another group of 2 month old SD rats was set up as young group. After 3-month intervention, the total number of cell crossing and the total behavior distance of rats were recorded through open field test. Rat serum reactive oxygen species (ROS) content, thymus index and spleen index were detected. Result: Compared with young group rats, the total number of cell crossing decreased in aging model group (P<0.01), the total action distance of natural aging rats decreased (P<0.05), serum ROS content increased (P<0.01), and thymus index and spleen index decreased (both P<0.01). Compared with the aging model group, the number of cell crossing and total action distance of all traditional Chinese medicine groups and resveratrol group increased (P<0.05). The serum content of ROS decreased (P<0.05). Traditional Chinese medicine high-dose group and resveratrol group showed increase in the thymus index and spleen index (P<0.01), the traditional Chinese medicine medium-dose group showed increase in the rat thymus index (P<0.05). Conclusion: The extracts from GNC can improve the mental retardation state of natural aging rats and enhance their space exploration capacity, improve the function of immune organs and reduce the serum ROS content of aging rats.