| Abstract: | ![]()
Ten adult male volunteers were studied to examine the effect on plasma beta-endorphin (B-EN) of bicycling and running for 1 h at 60% VO2max. All subjects were physically active and accustomed to both exercise modes (mean VO2max in ml X kg-1 X min-1; bicycling, 54; running, 59). Following preliminary VO2max tests, subjects participated in randomly ordered experimental sessions of bicycling and running for 1 h at loads requiring 60% of their mode-specific VO2max. Five subjects also participated in control sessions. Blood samples were collected immediately pre- and immediately post-exercise, and hematocrits were determined. Samples were centrifuged, separated, and plasma was stored at -50 degrees C until analysis for B-EN. Analysis involved separation of B-EN from beta-lipotropin by short column chromatography followed by radioimmunoassay. Despite an observed trend for elevated B-EN following exercise, there were no significant pre- to post-exercise differences (P greater than 0.05) in mean B-EN levels in any of the three sessions. Expressed as percentage change in B-EN, there were no significant differences between bicycling, running, or control. These results indicate that 1 h bicycling or running exercise at 60% VO2max does not consistently increase B-EN, and that responses are variable between individuals. |
