| 单纯疱疹病毒实时PCR检测以及与巢式PCR和病毒培养方法的比较 |
| |
| 引用本文： | 张修发,张萍,祝爱霞,江凡,蔡叶琴.单纯疱疹病毒实时PCR检测以及与巢式PCR和病毒培养方法的比较[J].深圳中西医结合杂志,2010,20(4):193-196,203. |
| |
| 作者姓名： | 张修发 张萍 祝爱霞 江凡 蔡叶琴 |
| |
| 作者单位： | 深圳市宝安区妇幼保健医院,广东,深圳,518133 |
| |
| 基金项目： | 深圳市宝安区科技局立项课题 |
| |
| 摘 要： | 目的：在Lightcycler系统上建立和评价HSV-1、HSV-2型病毒的实时定量PCR方法,并与巢式PCR和病毒分离培养的结果进行比较。方法：120例从疑似单纯疱疹病毒感染患儿口腔黏膜及唇黏膜取样,进行实时PCR,巢式PCR和病毒分离检测HSV-1、HSV-2的感染情况。结果：HSV-1阳性率：病毒分离和巢式PCR分别为34/120（28.3%）和36/120（30%）,实时PCR为38/120（31.7%）;HSV-2的检测：病毒分离阳性率为5/120（4.2%）,巢式PCR阳性率为7/120（5.8%）,实时PCR阳性率为8/120（6.7%）。结论：实时PCR增加了单纯疱疹病毒DNA检出率,尤其是和病毒分离相比。实时PCR和巢式PCR在敏感性上没有显著性差异。实时PCR技术具有快速扩增,降低污染风险的优势,它是诊断单纯疱疹病毒感染的一种合适方法。
|
| 关 键 词： | 单纯疱疹病毒Ⅰ型 单纯疱疹病毒Ⅱ型 实时PCR 巢式PCR |
Detection of Herpes Simplex Virus with Real-time PCR and Comparision with the Method of Nested PCR and Virus Isolation |
| |
| ZHANG Xiu-fa,ZHANG Ping,ZHU Ai-xia,JIANG Fan,CAI Ye-qing.Detection of Herpes Simplex Virus with Real-time PCR and Comparision with the Method of Nested PCR and Virus Isolation[J].Shenzhen Journal of Integrated Traditional Chinese and Western Medicine,2010,20(4):193-196,203. |
| |
| Authors: | ZHANG Xiu-fa ZHANG Ping ZHU Ai-xia JIANG Fan CAI Ye-qing |
| |
| Institution: | ZHANG Xiu-fa,ZHANG Ping,ZHU Ai-xia,JIANG Fan,CAI Ye-qing (Shenzhen Bao'an Maternal and Child Health Hospital,Guangdong Shenzhen 518133) |
| |
| Abstract: | Objective To set up and evaluate HSV-1,HSV-2 qualitative real-time PCR on the Lightcycler system and to compare the results with those of the 'in-house' nested PCR and virus isolation.Methods 120 consecutive samples from herpes lips lesions from children suspected of having HSV infections were tested with real-time PCR,nested PCR and virus isolation.Results 34 and 36 samples (28.3% and 30%) were positive for HSV-1 by virus isolation and nested PCR,whereas 38 (31.7%) were positive by real-time PCR.HSV-2 was ... |
| |
| Keywords: | HSV-1 HSV-2 Real-time PCR Nest-PCR |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
