Restoration of tumour suppressor hsa-miR-145 inhibits cancer cell growth in lung adenocarcinoma patients with epidermal growth factor receptor mutation |
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| Authors: | William C.S. Cho Andrew S.C. Chow Joseph S.K. Au |
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| Affiliation: | 1. College of Information Science and Engineering, Hunan University, Changsha, Hunan 410082, China;2. National Super-computer Center in Changsha, Hunan University, Changsha, Hunan 410082, China;3. Education Technology and Computer Center, Zhaoqing University, Zhaoqing, Guangdong 516061, China;4. Department of Computer Science, Zagazig University, Zagazig, Sharkia 44519, Egypt;1. Department of Pathology, Kyung Hee University Hospital at Gangdong, Kyung Hee University School of Medicine, Seoul, South Korea;2. Department of Pathology, Graduate School of Medicine, Kyung Hee University, Seoul, South Korea;3. Department of Pathology, Kyung Hee University Hospital, Kyung Hee University, Seoul, South Korea;4. Department of Internal Medicine, Graduate School of Medicine, Kyung Hee University, Seoul, South Korea;1. Lab of Oncogenomics, Department of Pathology, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea;2. Functional RNomics Research Center, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea;3. Cancer Evolution Research Center, College of Medicine, The Catholic University of Korea, Seoul, Republic of Korea;4. Department of Biochemistry, College of Medicine, The Catholic University of Korea, Seoul 137-701, Republic of Korea;5. Techno-Art Division, Underwood International College, Yonsei University, Seoul, Republic of Korea;1. Department of Pathology, Zhejiang University School of Medicine, Hangzhou 310058,China;2. State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Zhejiang University, Hangzhou, China;3. Department of Chemotherapy, Zhejiang Provincial People’s Hospital, Hangzhou, China;4. Department of Surgical Oncology, Anqing Municipal Hospital, Anqing, China;5. Department of Neurosurgery & Physiology, LSU Health Science Center, Shreveport, LA, United States of America;6. Department of Surgical Oncology, The First Affiliated Hospital, Zhejiang University, Hangzhou, China |
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| Abstract: | BackgroundIn Hong Kong, about 30% of non-small cell lung cancer patients have never smoked tobacco. Among women, 83% are never-smokers and their histological type is invariably adenocarcinoma with 70% incidence of epidermal growth factor receptor (EGFR) mutation. The present study focuses on the microRNA (miRNA) expression profiles of this important subset of lung cancer.MethodsPaired samples collected from the lung cancer tissue and adjacent normal lung parenchyma of 10 non-smoking patients with lung adenocarcinoma were profiled by miRNA microarray. Results were validated by quantitative reverse transcription polymerase chain reaction. Transfected cell viability assays were applied to determine the effects of candidate miRNAs on lung cancer cells.ResultsComparing paired lung cancer tissue with adjacent normal lung parenchyma, hsa-miR-1261, hsa-miR-145, hsa-miR-21, hsa-miR-182, hsa-miR-183 and hsa-miR-210 were found to be the most differentially expressed miRNAs. Most interestingly, an obvious inhibition of cell growth was observed in the EGFR mutant lung adenocarcinoma after transfection of hsa-pre-miR-145.ConclusionsOur study is the first report to connect miR-182 to lung cancer. Our results also show that restoration of tumour suppressor hsa-miR-145 inhibits cancer cell growth in EGFR mutant lung adenocarcinoma. Further study on these specific differentially expressed miRNAs may provide important information on peculiar tumourigenetic pathways and may identify useful biomarkers. |
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