| 615系小鼠可移植性肝癌中H-ras基因的表达 |
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| 引用本文： | 邹长华,蓝天鹤,孙芝琳,徐国威,顾健人. 615系小鼠可移植性肝癌中H-ras基因的表达[J]. 四川大学学报(医学版), 1988, 0(3) |
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| 作者姓名： | 邹长华 蓝天鹤 孙芝琳 徐国威 顾健人 |
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| 作者单位： | 基础医学院生物化学教研室(邹长华，蓝天鹤，孙芝琳)，上海市肿瘤研究所生化与分子生物学室(徐国威)，上海市肿瘤研究所生化与分子生物学室(顾健人) |
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| 基金项目： | 高等学校博士学科点专项基金 |
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| 摘 要： | 作者用盐酸胍法从615系小鼠原发性可移植性肝癌(H651、H22)实体瘤组织及正常615系小鼠肝组织(N615)中制备总RNA,经Oligo(dT)纤维素亲和层析法纯化得到 poly(A)~+RNA,再经兔网织红血球溶胞物体系测活及电泳鉴定后,作Northern 印迹和经α-~(32)P-标记的H-ras、Ki-ras及C-myc癌基因分子探针杂交,发现:仅H615中有H-ras基因的明显表达信号,N615中仅有弱表达。
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| 关 键 词： | 615系小鼠肝癌poly(A)~+RNA Northern印迹 分子杂交 H-ras基因表达 |
EXPRESSION OF H-ras GENE IN MOUSE 615 TRANSPLANTABLE HEPATOMA |
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| Zou Changhua,Lang Tianhe,Sun Zhilin. EXPRESSION OF H-ras GENE IN MOUSE 615 TRANSPLANTABLE HEPATOMA[J]. Journal of Sichuan University. Medical science edition, 1988, 0(3) |
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| Authors: | Zou Changhua Lang Tianhe Sun Zhilin |
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| Affiliation: | Zou Changhua;Lang Tianhe;Sun Zhilin Department of Biochemistry Xu Guowei;Gu Jianren Department of Biochemistry and Molecular Biology Shanghai Cancer Institute |
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| Abstract: | Poly (A)~+ RNA from mouse 615 hepatoma(H615, H22) and normal mouse 615 liver(N615) was isolated by guanidinium chlorideand the Oligo (dT) cellulose affinity chro-matography method. RNA was denaturedby the formaldehyde-formamide methodand performed with the agarose electro-phoresis. After the Northern blot transferthe nitrocellulose filters were hybridizedwith ~(32)P-labeled H-ras, N-ras, Ki-ras andC-myc probes. The results showed thatthere was an expression of H-ras in H615,but a very weak expression in N615. |
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| Keywords: | Poly (A)~+ RNA of mouse 615 hepatoma Northern blot transfer Hybridization H-ras gene expression |
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