天麻总提物对小鼠学习记忆能力的影响

目的观察天麻总提物对小鼠学习记忆能力的获得、巩固、再现功能的影响,为临床研发新药提供依据。方法昆明种雄性小鼠随机分为正常组、模型组、对照组、天麻总提物组,各给药组给予相应药物灌胃,灌胃容积为0.2 mL/10 g,正常组与模型组给予等容积蒸馏水灌胃,连续16 d。于给药11 d后复制东莨菪碱、氯霉素、乙醇致小鼠记忆获得、巩固、再现功能障碍模型,采用Morris水迷宫进行定位航行实验及空间探索实验,测定小鼠逃避潜伏期和空间搜索距离,以及原平台象限时间百分比和距离百分比。结果天麻总提物能明显缩短模型小鼠逃避潜伏期及搜索距离(P0.05,P0.01),显著增加原平台象限时间和距离百分比(P0.01)。结论天麻总提物能具有改善模型小鼠记忆获得、促进记忆巩固及记忆再现的作用。

Effects of Extractive from Gastrodiae Rhizoma on Learning-Memory Function in Mice

Objective To evaluate the effects of extractive from Gastrodiae Rhizoma on acquisition, consolidation and retrieval of learning-memory function in mice; To provide some reference for clinical research and development of new drugs. Methods Male Kunming mice were randomly divided into control group, model group, positive control group and Gastrodia extractive group. Positive control group and Gastrodia extractive group were given gavage by using relevant medicine 0.2 mL/10 g, and the control group and model group were given gavage with the same amount of distiUed water for 16 days. After receiving gavage for continuous 11 days, memory acquisition barrier model was induced by scopolamine; memory consolidation barrier model was induced by chloromycetin; memory retrieval barrier model was induced by EtOH. The learning-memory function was reviewed by escape latency and spatial search distance. The quadrant and distance search time percentage was detected through directional navigation test and spatial probe test in Morris water maze. Results Extractive from Gastrodia Rhizoma shortened the time for acquisition, consolidation and retrieval of learning memory about escape latency and spatial search distance （P 〈 0.05, P 〈 0.01）, and the quadrant and distance search time percentage were prolonged （P〈0.01）. Conclusion Extractive from Gastrodia Rhizoa can effectively improve the acquisition, consolidation and retrieval of learning-memory function in mice.