蛇葡萄素脂质体的制备研究

目的:优选蛇葡萄素脂质体的处方和制备工艺,并评价其质量。方法:采用薄膜-超声法制备蛇葡萄素脂质体及冻干粉,以包封率、表面形态和粒径为检测指标,利用均匀试验法优选出制备蛇葡萄素脂质体的最佳工艺条件。用显微镜法观察其形态,粒度测定仪测量粒径和表面电荷。采用透析法测定包封率和渗漏率,紫外分光光度法测定蛇葡萄素含量。结果:脂质体呈典型单室,近球型,大小相近,分散均匀,平均粒径为(258.2±51.2)nm,表面电荷19.0 mV。包封率达到62.3%,磷脂氧化指数为0.83%。结论:该脂质体处方和制备工艺稳定可行。

Study on preparation of ampelopsin liposomes

Objective: To study the formulation and preparation of ampelopsin liposomes and evaluate their quality.Method: The liposomes were prepared by a film-ultrasonic dispersion technique.Served as quota with the entrapment ratio and appearance and diameter of the liposomes,the optimal formulation and preparation were selected by means of an uniform design test.The appearance of liposomes was observed by micrography.The diameter and electric charge of surface were determined by granularity mensuration instrument.The entrapment ratio and the leakage rate of ampelopsin liposome were determined by means of dialyze.The content of ampelopsin was determined by UV.Result: The result of electron micrography and the size distribution showed that the liposomes were similar to spherical small unilamellar vesicles.The mean diameter was(258.2±51.2) nm and the electric charge of surface is 19.0 mV.The entrapment ratio of ampelopsin liposomes was 62.3% and the lecithoid oxidative rate was 0.83%(n=3).Conclusion: The selected formulation and preparation of ampelopsin liposomes is efficient and practicable.