邻苯二甲酸二丁酯对雄性果蝇生殖能力影响及其遗传毒性

目的：研究邻苯二甲酸二丁酯（di—n-butyl phthalate，DBP）对雄性果蝇生殖能力的影响及其遗传毒性．方法：①将未交配雄性果蝇随机分为5组，分别给予含DBP为0，1，4，16，64mg／g的培养基喂饲，10d后取存活的实验雄蝇进行交配试验和生育试验．②将雄性果蝇随机分为4组，分别喂饲含DBP为0，50，200，800mg／L的溶液，采用伴性隐性致死（SLRL）试验，观察DBP对雄性果蝇生殖细胞的致突变作用．结果：染毒10d后随着DBP浓度升高各染毒组雄蝇1h交配率和子代发生量逐渐降低，64mg／g组分别为40％和486±265，明显低于对照组的85％和861±312（P〈0．05）；生育期1—10d各染毒组的不育率均显著高于对照组（P〈0．01），10d后只有较高浓度组的不育率高于对照组（P〈0．05）；50mg／L浓度组精母细胞Ⅲ阶段的SLRL突变率（0．990％）显著高于对照组（0．106％，P〈0．01）．结论：DBP对性成熟的雄性果蝇能造成一定的生育障碍和遗传损伤．

Effects of Dibutyl phthalate on procreant ability and its genotoxity in male Drosophila melanogaster

AIM： To study the effects of dibutyl phthalate （DBP） on the procreant ability and its genotoxity in male Drosophila melanogaster. METHODS：（1）The non-copulated male Drosophila melanogasters were divided randomly into 5 groups and were continuously fed with medium containing 0,1,4,16,64 mg/g DBP for 10 days. Then the treated male flies were brought to the copulation trial and the reproduction trial. （2）The male Drosophila melanogasters were divided randomly into 4 groups and were continuously fed with solution containing 0,50,200,800 mg/L DBP for 3 days. The mutation-inducing effects of DBP was observed using a Sex-Linked Recessive Lethal Test （SLRL test） in Dro- sophila melanogaster. RESULTS：The mating rate in 1 hour and the average reproduction of male flies in DBP groups were gradual- ly decreased with the rising of DBP concentration. The mating rate in 1 hour （ 40% ） and the average reproduction （ 486 ± 265 ） of male flies in 64 mg/g DBP group were lower significantly than those in control group respectively 85% , （ 861 ± 312 ） , P 〈 0.05]. During the first 10 days of the procreation period, the yield rate of male flies in DBP groups were obviously higher as compared with control group （ P 〈 0. 01 ）. After continuously procreating for 10 days, the yield rate of male flies merely in higher concentration of DBP groups were significantly higher than that of control group（ P 〈 0.05 ）. The mutation rates （0. 990% ） of DBP at 50 mg/L concentration in third brood were significantly higher as compared with control group （ 0. 106%, P 〈 0.01 ）. CONCLUSION： DBP might reduce the procreant ability and bring out genotoxity effect on male Drosophila melanogaster.