内毒素对血管内皮细胞分泌功能的影响

目的　探讨内毒素对脐静脉内皮细胞分泌功能的影响。方法　选择体外培养的人脐静脉血管内皮细胞（ＨＵＶＥＣ）为研究对象，分别用内毒素、Ｌ左旋精氨酸和硝基左旋精氨酸进行处理，并检测上清液中内皮素１（ＥＴ１）和一氧化氮（ＮＯ） 含量。结果　内毒素使ＥＴ１ 和ＮＯ 含量增加；ＮＯ 则使ＥＴ１的含量降低。结论　内毒素能导致人脐静脉内皮细胞损伤，使ＥＴ１ 和ＮＯ的合成和释放增加；ＮＯ则抑制ＥＴ１ 的合成和释放。

Effects of endotoxin on the secretive function of the human umbilical vein endothelial cells

Objective To study the effects of endotoxin on the secretive function of the human umbilical vein endothelial cells. Methods After the in vitro cultured human umbilical vein endothelial cells (HUVEC) were handled with endotoxin, L arginine and nitric L arginine respectively, the contents of ET 1 and nitric oxide (NO) in supernatant were determined. Results Endotoxin could increase the synthesis and release of ET 1 and NO.NO could decrease the synthesis and release of ET 1. Conclusion Endotoxin might result in the damage of endothelial cells, therefore the overproduction of ET 1 and NO were induced by endotoxin. NO could inhibit the synthesis and release of ET 1.;