大鼠肾小管上皮细胞的原代培养及传代

建立大鼠肾小管上皮细胞原代培养、传代及鉴定方法。方法采用研磨、消化培养法，并以０．２５％胰蛋白酶（Ａ组）、０．２５％胰蛋白酶－０．０２％ＥＤＴＡ（Ｂ组）分别消化、传代、对比，以免疫细胞化学方法鉴定细胞种类。结果成功培养、传代（１３～１８代）并鉴定（抗Ｃｙｔｏｋｅｒａｔｉｎ１８抗体）了肾小管上皮细胞，发现Ａ组消化传代效果明显优于Ｂ组。结论０．２５％胰蛋白酶消化传代方法是大鼠肾小管上皮细胞原代培养、传代及鉴定的有效手段。

primary culture and passage of rat kidney tubular epithelial cells in rats

Objective To establish a method for primary culture, passage and identification ofrat renal tubular epithelial cells. Method Grinding and digesting were used in primqry culture, and0.25% tryspase (group A) and 0. 25% tryspase-0.02% EDTA (group B) were respectively used inpassage. The cell types were identified by immunocytochemistry. Result The primary and secondaryculture of renal tubular epithelial cells were both successful (the passage number could reach 13-18), sowas the identification of the cultured cells. The passage effect of group A was significantly better thanof group B. Conclusion The present method (group A) is an efficient means for the primary culture,passage and identification of rat kidney tubular epithelial cells.