醛缩酶同工酶琼脂糖电泳测定法

建立分离血清醛缩酶同工酶的方法。利用不连续缓冲体系琼脂糖凝胶电泳进行分离，ＡＬＤ－ＧＬＡＰＤ酶偶联反应与ＰＭＳ－四唑盐递氢呈色系统来显示酶活性区带。２０名健康成人血清醛缩酶同工酶电泳，结果显示两条清晰的酶活性区带ＡＬＤ－Ａ和ＡＬＤ－Ｂ；同时，实验条件的研究也显示：在ｐＨ８．２、终末反应物浓度ＦＤＰ７ｍｍｏｌ／Ｌ、ＮＡＤ５ｍｇ／Ｌ和ＧＬＡＰＤ３Ｕ／Ｌ时区带显色最佳。该方法简便、可靠、对临床肝癌及肝硬化的诊断有一定的辅助价值。

Assay of aldolase isoenzymes with agarose gel electroph oresis

A new electrophoretic method to separate ALD isoenzymes on agarose gel by using a discontinuous buffer system. have been developed Its principle is based on the ALD GLAPD enzyme coupled reaction and the reduction of tetrazolium salt (INT) to a colored formazan. Serum samples from 20 healthy adults were assayed by the method. The results showed two distinct enzymatic bands, ALD A and ALD B. In addition, most suitable reaction conditions for the assay were also studied. Optimal pH was 8 2, Optimal concentrations in the reaction system were FDP 7 mmol/L, NAD 5mg/L, GLAPD 3U/L. At last, the primary clinical application of the assay was studied. The results suggested that the assay of ALD Isoenzymes in serum may be useful in the diagosis of hepafic cancer and liver cirrhosis.