Identification of maxicircle DNA sequences in Leishmania Tarentolae that are homologous to sequences of specific yeast mitochondrial structural genes |
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| Authors: | L Simpson T W Spithill A M Simpson |
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| Affiliation: | Biology Department and Molecular Biology Institute, Los Angeles, CA 90024, U.S.A. |
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| Abstract: | ![]()
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| Keywords: | maxicircle DNA kinetoplast DNA heterologous probes SSC, 0.15 M NaCl/0.015 M sodium citrate, pH 7 HHM-5, 1 M NaCl, 10 mM Tris-HCl (pH 7.4), 0.2% bovine serum albumin, 0.2% Ficoll, 0.2% polyvinylpyrolidone, 5% formamide HMA, 5×SSC, 0.2% sodium dodecyl sulfate (SDS), 0.5 mg/ml sonicated denatured salmon sperm DNA, 0.5 mg/ml poly(rA), 0.02% bovine serum albumin, 0.02% Ficoll, 0.02% polyvinylpyrolidone, 50% formamide |
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