| 慢性缺氧和二氧化碳潴留大鼠脑阴离子转运蛋白的研究 |
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| 引用本文： | 张献全,钱桂生,范卫东. 慢性缺氧和二氧化碳潴留大鼠脑阴离子转运蛋白的研究[J]. 中国病理生理杂志, 1999, 15(1): 76-78 |
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| 作者姓名： | 张献全 钱桂生 范卫东 |
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| 作者单位： | 第三军医大学附属新桥医院,呼吸病研究所,重庆,400037 |
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| 摘 要： | 目的：观测慢性缺氧和二氧化碳潴留大鼠脑桥组织中脑阴离子转运蛋白（ＡＥ３）ｍＲＮＡ的变化。方法：采用密闭舱内灌注混合气体的方法,分别复制慢性缺氧,慢性缺氧伴二氧化碳潴留及慢性呼吸性酸中毒大鼠模型,以地高辛标记的（ＡＥ３）ｃＤＮＡ探针,应用点杂交技术,对各实验组及对照组大鼠脑桥组织中ＡＥ３ｍＲＮＡ的相对含量进行测定。结果：（１）慢性缺氧组ＡＥ３ｍＲＮＡ的相对含量明显地低于对照组（Ｐ〈０．０５）,慢性缺
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| 关 键 词： | 缺氧 肺性脑病 二氧化碳 AE3 mRNA 大鼠 |
Chronic hypoxia and hypercapnia on the expression of anion exchanger mRNA in rat brains |
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| ZHANG Xian-Quan,QIAN Gui-Sheng,FAN Wei-Dong. Chronic hypoxia and hypercapnia on the expression of anion exchanger mRNA in rat brains[J]. Chinese Journal of Pathophysiology, 1999, 15(1): 76-78 |
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| Authors: | ZHANG Xian-Quan QIAN Gui-Sheng FAN Wei-Dong |
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| Abstract: | AIM:To study the expression of anion exchanger (AE 3) of brain in rats under chronic hypoxia and hypercapnia.METHODS:With a sensitive digoxigenin labelled AE 3 cDNA probe,dot blot was used to evaluated changes of AE 3 mRNA in rats models of chronic hypoxia,chronic hypoxia with hypercapnia and chronic acidosis established by pouring mixed gases into the chamber respectively as compared with control.RESULTS:It was found that the relative contents of AE 3 mRNA in rat brains in experimental group were significantly lower than that in control group( P <0 05),and the relative contents of AE 3 mRNA in rat brains of hypoxia with hypercapnia and chronic acidosis were also significantly lower than that of chronic hypoxia( P <0 01),but there was no significant difference between chronic hypoxia with hypercapnia group and chronic acidosis group( P >0 05).CONCLUSION:AE 3 may play an important role in the respiratory failure and pulmonary encephalophathy. |
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| Keywords: | Anoxia Hypercapnia Lung Brain |
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