HPLC同时测定复方益肝灵片中水飞蓟宾、五味子醇甲的含量

目的 建立用HPLC同时测定复方益肝灵片中水飞蓟宾、五味子醇甲的含量。方法Diamonsil C18色谱柱（250mm×4．6mm，5μm），流动相为甲醇一水，梯度洗脱为0—60min（甲醇40％→75％，水60％→25％），检测波长：水飞蓟宾287nm，五味子醇甲250nm。结果水飞蓟宾和五味子醇甲分别在4．96～39．68μg·mL^-1（r=0．9998）和4．00～32．00μg·mL^-1（r=0．9998）内呈良好的线性关系，平均加样回收率分别为98．8％和99．5％，RSD分别为0．96％和0．59％。结论该方法简便、快速、重复性好，可用于复方益肝灵片的质量控制。

Simultaneous Determination of Silymarin and Schizandrol in Compound Yi Ganling Tablets by HPLC

OBJECTIVE To simultaneous determine the content of silymarin and schizandrol in compound Yi Ganling tablets by HPLC. METHODS The analytical column was a Diamonsil C18 column （250 mm ×4. 6 mm,5 μm）. The mobile phase consisted of methanol - water. The gradient condition was 0→60 min, methanol 40%±75% and water 60%→25%. The detection wavelengths of silymarin and scbizandrol were at 287 nm and 250 nm. RESULTS The calibration curves of silymarin and schizandrol were in good linearity over the ranges of 4. 96 - 39.68 μg · mL - ^-1（ r = 0. 999 8 ） and 4. 00 - 32.00 μg · mL^-1 （ r = 0. 999 8 ） , the average recoveries were 98. 8% and 99. 5% ,RSD were 0. 96% and 0. 59%. CONCLUSION The method is simple, rapid, reliable and can be used for the drug control.