终末期肾衰竭维持血液透析患者的血清代谢组学研究

目的:采用气相色谱-质谱联用(GC/MS)为基础的代谢组学技术分析终末期肾病(end stage renal disease,ESRD)维持血液透析患者血清中代谢物的变化,筛选出可用于ESRD早期临床诊断的潜在生物标志物,探讨ESRD血液透析患者代谢紊乱的发生机制.方法:采用GC/MS检测63例ESRD患者(病例组)和...

Serum metabolomics of patients with end-stage renal failure maintained on hemodialysis

OBJECTIVE To explore the changes of metabolites in sera of patients with end-stage renal disease(ESRD) maintained on hemodialysis using gas chromatography-mass spectrometry(GC/MS) metabolomics to search for potential biomarkers related to ESRD and elucidate the mechanism of ESRD metabolic disorders.METHODS GC/MS was employed for detecting the metabolites in sera of 63 ESRD patients(case group) and 20 matched healthy people(control group).Supervised partial least squares discrimination analysis(PLS-DA) was utilized for analyzing the serum metabolic profiles of samples to search for differential metabolites between two groups.RESULTS The metabolic profiles of control and case group were well-distinguished.And 34 differential metabolites were identified through the databases of NIST,Wiley Registry metabolome and standards.The ROC curve of 34 differential metabolites was plotted and the value under the curve was calculated.Substances with an area greater than 0.5 included 28 metabolites of hydroxylamine,urea and fumaric acid,etc.CONCLUSION ESRD patients have metabolic abnormalities of energy metabolism,amino acid metabolism,lipid metabolism,urea cycle and purine metabolism.Hydroxylamine,urea and fumaric acid may be potential diagnostic markers for ESRD.