不同浓度法舒地尔促进皮肤创面愈合作用的研究

目的：观察法舒地尔（HA1077）促进创面愈合的效果,并探讨HA1077与创面愈合的量效关系,寻找出促进创面愈合的最佳剂量。方法：18只Wistar大鼠背部左右两侧致直径为2cm的圆形皮肤缺损。随机3只动物6个创面为一组,分为6组,分别给予10、20、40、80和160μmol/L盐酸法舒地尔和生理盐水（对照组）创面喷洒,每个创面0.5ml,隔日创面追加喷洒。实验3、7、10d计算伤口面积,实验10d取创面组织,观察组织学变化。结果：各组大鼠创面面积随伤后时间延长而逐渐缩小,20μmol/L组创面面积明显小于同时间点其他各组,除实验7d与80μmol/L组创面面积比较差异无显著性外,其余各时间点差异均有显著性（P〈0.05）。病理学变化显示,创伤后10d,应用20μmol/L法舒地尔组创面新生肉芽组织生长及新生表皮生长速度明显优于其他各组。结论：HA1077可促进皮肤缺损创面愈合,以20μmol/LHA1077效果更为明显。

Effect of different dosage of fasudil on wound healing in rats

Objective：To observe the effect of fasudil （HA1077） on wound healing in rats, to analyze the doseeffect correlation, and to look for the best dosage of HA1077 for treatment. Methods：Two circular skin defects of 2 cm in diameter were prepared on two sides of the back in Wistar rats. Eighteen rats were randomly divided into six groups （3 rats in each group）, and 10, 20, 40, 80, 160 μmol/L HA1077 and 0.9% NaCl （control group） all in volume of 0.5 ml were respectively sprayed to each wound on the back, and it was repeated once for two days. The area of wound was estimated on days 3, 7, 10, and histological changes were observed on day 10. Results： The area of wounds reduced gradually after injury. The wound area on which 20 μmol/L HA1077 was applied was the smallest among the groups at the same time point. Except that on the 7 day there was no significant difference in wound area where 80 μmol/L HA1077 was used, the difference was significant（P〈0.05）. The pathological examination showed that growth of the granulation tissue and epidermis was better in 20 μmol/L HA1077 group than the other groups on the 10th day after injury. Conclusion： HA1077 can enhance wound healing of skin defect, especially when 20μmol/L HA1077 is used topically.