自噬相关蛋白 Beclin1、LC3在甲状腺癌合并颈部淋巴结转移中的表达及临床意义

目的:探究分析与细胞自噬相关的蛋白Beclin1及LC3在甲状腺癌合并颈部淋巴结转移中的表达及其在临床治疗中的意义。方法:采用免疫组织化学方法检测,选取48例我院2012年2月至2014年2月收集的甲状腺癌组织、30例癌旁正常组织用以检测Beclin1及LC3蛋白表达,另取38例甲状腺癌转移淋巴结以及30例的正常淋巴结临床标本,用以检测Beclin1及LC3蛋白表达。结果:在取材的所有样本中均有表达Beclin1,但在甲状腺癌组织中含量低于癌旁正常的甲状腺组织(P<0.01),转移淋巴结中Beclin1水平明显低于正常淋巴结组(P<0.01);免疫组化结果显示在所有样本中也检测到LC3蛋白表达,在甲状腺癌组织中的含量最低,并且显著低于癌旁甲状腺组织(P<0.01),在发生转移的淋巴结组织中,LC3含量低于正常淋巴结组织(P<0.01)。结论:自噬功能相关蛋白Beclin1与LC3的表达与甲状腺癌是否发生转移密切相关,是肿瘤向囊外扩展的直接标记。该蛋白具有作为甲状腺癌淋巴结转移的术前评估指标的潜在可能,具有一定的指导意义。

Expression of autophagy-related protein Beclin1 and LC3 in thyroid carci-noma with cervical lymph node metastases and its clinical significance

Objective:To investigate the autophagy-related protein Beclin1 and LC3 expression in thyroid carcinoma with cervi-cal lymph node metastasis and clinical significance.Methods:By immunohistochemistry assay,from our hospital in February 2012 to February 2014 48 cases of papillary thyroid carcinoma tissues and 30 cases of para -tumor normal tissues were gathered to detect ex-pression of Beclin1 and LC3,as well as another 38 cases of papillary thyroid cancer with lymph node metastasis and 30 cases of clinical samples of normal lymph nodes to detect Beclin1 and expression of LC3.Results:Beclin1 were expressed in all samples,being lower in thyroid cancer than adjacent normal thyroid tissue (P <0.01 ),metastatic lymph nodes Beclin1 level was significantly lower than that of the normal lymph node group (P <0.01 ).The results showed LC3 protein expression was detected in all samples,its expression was the lowest in thyroid papillary tissue,beingsignificantly lower than that of their surrounding thyroid tissue (P <0.01 )andin the event of metastasis lymph LC3 content was lower than that of in normal lymph nodes (P <0.01 ).Conclusion:Autophagy protein Beclin1 and LC3 expression are closely related to thyroid cancer metastasis,and tumor extracapsular extension of direct labeling.The proteins has a potential as a preoperative evaluation of lymph node metastasis of thyroid cancer,which has a certain significance.