幽门螺杆菌感染与胃癌组织中p27蛋白表达的关联性分析

［摘要］ 目的探讨幽门螺杆菌（Helicobacter pylori，H.pylori）感染与胃癌组织细胞周期调节因子p27蛋白表达的关联性，了解H.pylori感染引起胃癌的发病机制。 方法选取胃癌切除术的胃癌组织标本62例和癌旁组织标本42例。采用14C-尿素酶呼吸试验法检测癌组织标本H.pylori感染情况。应用免疫组织化学染色快捷法检测组织中p27蛋白表达水平。分析H.pylori感染与胃癌患者p27表达的关联性。 结果62例胃癌患者中H.pylori感染者40例（64.52%）。高、中分化胃癌患者p27蛋白表达率高于低分化胃癌患者（P＜0.01）；不同性别、年龄、肿瘤大小、TNM分期、肿瘤浸润深度及淋巴结胃癌患者p27蛋白表达率差异无统计学意义（P＞0.05）。 H.pylori感染与胃癌癌组织中p27蛋白表达呈负性相关（P＜0.01）。多因素Logistics回归分析，结果显示肿瘤分化程度是胃癌组织p27蛋白阳性表达的危险因素（P＜0.01）。p27在胃癌组织中的表达率明显低于癌旁组织（P＜0.01）。 结论H.pylori感染与胃癌患者p27蛋白表达水平存在关联性，H.pylori感染可能通过降低p27 蛋白表达水平引起胃黏膜癌变，p27蛋白可作为临床诊断胃癌及是否合并H.pylori感染的辅助指标，参与胃癌的发生发展，可作为疾病后期诊断、治疗及预后评估的重要指标。

Association between Helicobacter pylori infection and p27 protein expression in gastric cancer

［Abstract］ObjectiveTo investigate the correlation between Helicobacter pylori(H.pylori) infection and the expression of cell cycle regulator p27 protein in gastric cancer tissues in order to understand the pathogenesis of gastric cancer caused by H.pylori infection. 〖WTHZ〗MethodsSixty-two specimens of gastric cancer tissues undergoing gastric cancer resection were selected. The 14C-urease breath test was used to detect H.pylori infection in the specimens, and the immunohistochemical staining method was applied to measure the expression level of p27 protein, so as to analyze the correlation between H.pylori infection and p27 expression in gastric cancer patients. 〖WTHZ〗Results Forty cases in 62 patients with gastric cancer were infected by H.pylori(64.52%). The expression rate of p27 protein in gastric cancer tissues was closely related to the degree of tumor differentiation(P＜0.01), there was no correlation between the expression rate of p27 protein and the gender, age, tumor size, and TNM stage, tumor invasion depth and lymph node metastasis(P＞0.05). H.pylori infection was negatively correlated with p27 protein expression in gastric cancer tissues(P＜0.01). Logistics analysis showed the degree of tumor differentiation was the main factor of positive expression of p27 protein in cancer tissues(P＜0.01). Expression of p27 in gastric cancer tissues was lower than in paracaner tissues(P＜0.01). 〖WTHZ〗ConclusionThere is a certain correlation between H.pylori infection and the expression level of p27 protein in gastric cancer patients. H.pylori infection may cause gastric mucosal canceration by reducing the expression level of p27 protein, and p27 protein can be used for the clinical diagnosis of gastric cancer and the determination of whether it is associated with H.pylori infection. p27 protein is also an important indicator to the later diagnosis, treatment and prognosis evaluation of gastric cancer.