间歇性低氧预处理对大鼠心肌促血管生成作用的实验研究

目的:采用常压间歇性低氧(intermittent hypoxia,IH)大鼠的动物模型,观察间歇性常压低氧预处理的促血管生成作用。方法:成年雄性Wistar大鼠25只,体重210～215g,随机分为2大组:对照组(C组,n=5)和间歇性低氧预处理组(IH组,n=20)。IH组动物进行间歇性低氧预处理(4h/d,间歇缺氧1d者为IH1组,7d者为IH2组,14d者为IH3组,28d者为IH4组),按计划完成实验后测定心肌血管内皮生长因子(VEGF)蛋白表达及毛细血管密度。结果:间歇性低氧预处理大鼠心肌VEGF蛋白表达增加,心肌毛细血管密度增高。结论:间歇性低氧预处理能促进大鼠心肌内的血管生成,其机制可能与心肌VEGF表达增高有关。

The Angiogenesis Effect of Intermittent Hypoxia Precondition on Myocardium in Rats

Objective:To determine the effect of intermittent hypoxia(IH) precondition on myocardium by using a rat model.Methods:Twenty-five male adult Wistar rats were assigned into two groups randomly:control group(C group) and IH precondition group(IH group).The rats in IH group were exposed to intermittent hypoxia,for 4h day continuously.According to IH precondition-ing duration,the animals were subdivided into 1-day group(IH1),7-days group(IH2),14-days group(IH3) and 28-days group(IH4).After these experiments,the expression of vascular endothelial growth factor(VEGF) protein was detected in myocardium.Tissue sections were stained for alkaline phosphatase with indoxy-tetrazolium method to detect capillary density.Results:The expression of VEGF protein was increased in IH group significantly.Compared with C group,the capillary density in IH4 group was increased significantly.Conclusion:Angiogenesis in myocardium of rats can be induced by intermittent hypoxia precondition.