Identification and detection method for genetically modified papaya resistant to papaya ringspot virus YK strain |
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Authors: | Nakamura Kosuke Akiyama Hiroshi Ohmori Kiyomi Takahashi Yuki Takabatake Reona Kitta Kazumi Nakazawa Hiroyuki Kondo Kazunari Teshima Reiko |
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Affiliation: | National Institute of Health Science, Tokyo, Japan. |
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Abstract: | Unauthorized genetically modified (GM) papaya (Carica papaya LINNAEUS) was detected in a commercially processed product, which included papaya as a major ingredient, in Japan. We identified the transgenic vector construct generated based on resistance to infection with the papaya ringspot virus (PRSV) YK strain. A specific detection method to qualitatively monitor papaya products for contamination with the GM papaya was developed using the real-time polymerase chain reaction. |
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