N-乙酰半胱氨酸对缺氧缺血新生大鼠脑细胞间黏附分子-1及细胞凋亡的影响

目的探讨N-乙酰半胱氨酸(NAC)对缺氧缺血脑损伤(HIBD)大鼠脑细胞间黏附分子-1及细胞凋亡的影响。方法7d龄SD大鼠，90只，随机分成3组，(1)假手术组(Sham)(n=30)；(2)HIBD模型组(Control)(n=30)：HIBD模型后即刻腹腔注射等量生理盐水；(3)HIBD药物组(NAC)(n=30)：HIBD模型后即刻腹腔注射N-乙酰半胱氨酸(NAC)，200mg/kg。各组大鼠于处置后6h、12h、24h、48h、7d分别采用免疫组化法和Tunel法检测脑组织中细胞间黏附分子-1(ICAM—1)和细胞凋亡数目。结果HIBD模型组脑组织中ICAM—1的表达和细胞凋亡数与假手术组和HIBD药物组相比均升高，有显著性差异。结论NAC可抑制HIBD脑细胞ICAM-1的表达及脑细胞的细胞凋亡程度，NAC对缺氧缺血新生大鼠脑损伤有保护作用。

The Effect of N-acetylcysteine on ICAM-1 Expression and Apoptosis in Neonatal Rats Brain after Hypoxic and Ischemic Brain Damage

Objective To explore the effect of N-acetylcysteine (NAC) on intercellular adhesion molecule-1(ICAM-1) expression and apoptosis in neonatal rats brain after hypoxic and ischemic brain damage (HIBD). Methods Ninety 7-day old SD rats were divided into sham operative group, HIBD group and NAC treatment group, each group containing 30 animals. ICAM-1 expression and the apoptotic rate of brain cells were measureed at 6h, 12h, 24h, 48h and 7days after model establishment by immunohistochemistry and TUNEL, respectively. Results Compared with sham operative group and NAC treatment group, the number of ICAM-1 expression cells and apoptotic cells in the brain tissue significantly increased in HIBD group. Conclusion NAC could inhibit ICAM-1 expression and cell apoptosis in the brain with HIBD, and had the preventive effect on hypoxic and ischemic brain damage in neonatal rats.